Immobilization of Target-Bound Aptamer on Field Effect Transistor Biosensor to Improve Sensitivity for Detection of Uncharged Cortisol

Field effect transistor (FET) biosensors are capable of detecting various biomolecules, although challenges remain in the detection of uncharged molecules. In this study, the detection of uncharged cortisol was demonstrated by interfacial design using a technique to immobilize target-bound aptamers....

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Bibliographic Details
Published in:Denki kagaku oyobi kōgyō butsuri kagaku 2021/03/05, Vol.89(2), pp.134-137
Main Authors: HAYASHI, Hiroki, TOYAMA, Ryo, TAKIBUCHI, Ryota, HIDESHIMA, Sho, KUROIWA, Shigeki, KANEKO, Naoto, HORII, Katsunori, OHASHI, Keishi, MOMMA, Toshiyuki, OSAKA, Tetsuya
Format: Article
Language:English
Subjects:
Aptamer
Aptamers
Biomolecules
Biosensors
Conformational Change
Cortisol
Field Effect Transistor
Field effect transistors
Hormones
Immobilization
Semiconductor devices
Sensitivity
Steric hindrance
Transistors
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Summary:Field effect transistor (FET) biosensors are capable of detecting various biomolecules, although challenges remain in the detection of uncharged molecules. In this study, the detection of uncharged cortisol was demonstrated by interfacial design using a technique to immobilize target-bound aptamers. The target-bound aptamers, which formed a higher-order structure than target-unbound aptamers, expanded the distance between adjacent aptamers and reduced the steric hindrance to the conformational change. The density-controlled aptamers efficiently induced their conformational changes with the cortisol binding, which resulted in the improvement of the sensitivity of FET biosensors.
ISSN:1344-3542
2186-2451
DOI:10.5796/electrochemistry.20-00144