Studies on Adsorption Kinetics and Thermodynamics of Macroporous Resin for Rosmarinic Acid

• Published in: Journal of Oleo Science 2021, Vol.70(3), pp.439-451
• Main Authors: Zhang, Jin-Hua, Zhang, Min, Bai, Bao-Qing, Jia, Huai-Wang, Fan, San-Hong
• Format: Article
• Language: English
• Subjects: Adsorption; Germination; Kinetics; Low concentrations; macroporous resin; Resins; Rosmarinic acid; salt stress; Seeds; Thermodynamics
• ISSN: 1345-8957; 1347-3352
• DOI: 10.5650/jos.ess20305

This experiment treated perilla seeds with different concentrations of NaCl solution to enrich and purify their rosmarinic acid (RosA). The results showed that low concentrations of salt (0-20 mmol/L) promoted seed germination, while high concentrations (> 20 mmol/L) inhibited germination. When the salt concentration was 20 mmol/L, the germination rate was the highest. The content of RosA in germinated perilla seeds was 3.5 mg/g, which was 3.5 times as much as that in the seeds without germination. The RosA was purified using NK-109 macroporous resin and its adsorption kinetics, isotherms and thermodynamics were determined. The adsorption kinetics showed that the adsorption behavior of RosA in NK-109 resin conformed to the pseudo-second-order kinetic model. The model for RosA in the NK-109 resin exhibited Langmuir adsorption based on a spontaneous exothermic process according to its adsorption thermodynamics, which included both physical and chemical adsorption. The optimized process conditions were as follows: the loading concentration of 0.04 mg/mL, loading volume of 40 mL, 70% methanol as the eluent with the volume of 60 mL, and the purity of RosA was 42.1%.