Genome‐wide identification and expression analysis of detoxification efflux carriers (DTX) genes family under abiotic stresses in flax

• Published in: Physiologia plantarum 2021-04, Vol.171 (4), p.483-501
• Main Authors: Ali, Essa, Saand, Mumtaz Ali, Khan, Ali Raza, Shah, Jawad Munawar, Feng, Simin, Ming, Cai, Sun, Peilong
• Format: Article
• Language: English
• Subjects: Annotations; Cadmium; Detoxification; Efflux; Extrusion; Flax; Flax - genetics; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genome, Plant - genetics; Genomes; Linum usitatissimum; Metabolites; MicroRNAs; miRNA; Multigene Family; Phylogenetics; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Polymerase chain reaction; Pore size; Porosity; Protein structure; Regulatory sequences; Secondary metabolites; Stress, Physiological - genetics; Tertiary structure; Toxic substances
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1111/ppl.13105

The detoxification efflux carriers (DTX)/multidrug and toxic compound extrusion (MATE) transporters encompass an ancient gene family of secondary transporters involved in the process of plant detoxification. A genome‐wide analysis of these transporters was carried out in order to better understand the transport of secondary metabolites in flaxseed genome (Linum usitassimum). A total of 73 genes coding for DTX/MATE transporters were identified. Gene structure, protein domain and motif organization were found to be notably conserved over the distinct phylogenetic groups, showing the evolutionary significant role of each class. Gene ontology (GO) annotation revealed a link to transporter activities, response to stimulus and localizations. The presence of various hormone and stress‐responsive cis‐regulatory elements in promoter regions could be directly correlated with the alteration of their transcripts. Tertiary structure showed conservation for pore size and constrains in the pore, which indicate their involvement in the exclusion of toxic substances from the cell. MicroRNA target analysis revealed that LuDTXs genes were targeted by different classes of miRNA families. Twelve LuDTX genes were chosen for further quantitative real‐time polymerase chain reaction analysis in response to cold, salinity and cadmium stress at 0, 6, 12 and 24 hours after treatment. Altogether, the identified members of the DTX gene family, their expression profile, phylogenetic and miRNAs analysis might provide opportunities for future functional validation of this important gene family in flax.