Neuropsin (Opn5) detection in the brain tissue of a murine model using long period fiber grating (LPFG)

•Neuropsin detected in brain tissue of a murine model by a long-period fiber grating.•High selectivity is revealed by immobilization of polyclonal antibodies on an LPFG.•PCA confirms that an LPFG biosensor is self-assembled and opsin is detected.•PC1 related to detection stage and PC2 to time during...

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Bibliographic Details
Published in:Optics and laser technology 2021-07, Vol.139, p.106972, Article 106972
Main Authors: Delgado-Macuil, R., González-León, K., Beltrán-Pérez, G.
Format: Article
Language:English
Subjects:
Antibodies
Biological recognition element
Biomarkers
Biosensor
Biosensors
Brain
Fourier transforms
Gratings (spectra)
Homogeneity
Long period fiber grating
Opsin detection
Principal components analysis
Self-assembly
Signal processing
Signal transmission
Structural and microscopic characterization
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Summary:•Neuropsin detected in brain tissue of a murine model by a long-period fiber grating.•High selectivity is revealed by immobilization of polyclonal antibodies on an LPFG.•PCA confirms that an LPFG biosensor is self-assembled and opsin is detected.•PC1 related to detection stage and PC2 to time during biosensor self-assembly. This paper presents the development of a biosensor based on long period fiber grating, using a polyclonal antibody (OPN5) as a biological recognition element, which enables the detection of opsin (Opn5) in the brain tissue of a murine model with high specificity. Opsin (Opn5) is overexpressed in brain, ovarian, breast and prostate carcinomata and it may function as an important new biomarker to diagnose and monitor several types of cancer. In this study, the fiber surface was chemically prepared for antibody immobilization. The structural homogeneity of the biosensor was characterized by Fourier-transform infrared spectroscopy at each stage of the self-assembling process, and the response changes were measured by a signal transmission at the biosensor output. The biosensor developed was tested to detect Opn5 in brain tissue, and the interaction of Opn5 was observed through the evolution of the transmission spectrum in real time. Finally, the transmission data were analyzed by a principal component analysis (PCA).
ISSN:0030-3992
1879-2545
DOI:10.1016/j.optlastec.2021.106972