Paper-based immune-affinity arrays for detection of multiple mycotoxins in cereals

• Published in: Analytical and bioanalytical chemistry 2018-03, Vol.410 (8), p.2253-2262
• Main Authors: Li, Li, Chen, Hongpu, Lv, Xiaolan, Wang, Min, Jiang, Xizhi, Jiang, Yifei, Wang, Heye, Zhao, Yongfu, Xia, Liru
• Format: Article
• Language: English
• Subjects: Affinity; Analytical Chemistry; Animals; Antibodies; Antibodies, Immobilized - chemistry; Arrays; Biochemistry; Cereals; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Contamination; Copolymerization; Crosslinking; Deoxynivalenol; Detection limits; Edible Grain - chemistry; Equipment Design; Food; Food Analysis - instrumentation; Food Analysis - methods; Food Contamination - analysis; Food Science; Food testing; Fungi; Grain products; Health aspects; Humans; Immune system; Immunoassay - instrumentation; Immunoassay - methods; Laboratory Medicine; Limit of Detection; Luminescent Measurements - instrumentation; Luminescent Measurements - methods; Methods; Monitoring/Environmental Analysis; Monoclonal antibodies; Mycotoxins; Mycotoxins - analysis; Photolithography; Polyethylene glycol; Polymerization; Reproducibility; Reproducibility of Results; Research Paper; T-2 Toxin - analogs & derivatives; T-2 Toxin - analysis; Toxins; Trichothecenes - analysis; Zearalenone; Zearalenone - analysis
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-018-0895-z

Mycotoxins produced by different species of fungi may coexist in cereals and feedstuffs, and could be highly toxic for humans and animals. For quantification of multiple mycotoxins in cereals, we developed a paper-based mycotoxin immune-affinity array. First, paper-based microzone arrays were fabricated by photolithography. Then, monoclonal mycotoxin antibodies were added in a copolymerization reaction with a cross-linker to form an immune-affinity monolith on the paper-based microzone array. With use of a competitive immune-response format, paper-based mycotoxin immune-affinity arrays were successfully applied to detect mycotoxins in samples. The detection limits for deoxynivalenol, zearalenone, T-2 toxin, and HT-2 toxin were 62.7, 10.8, 0.36, and 0.23 μg·kg -1 , respectively, which meet relevant requirements for these compounds in food. The recovery rates were 81–86% for deoxynivalenol, 89–117% for zearalenone, 79–86% for T-2 toxin, and 78–83% for HT-2 toxin, and showed the paper-based immune-affinity arrays had good reproducibility. In summary, the paper-based mycotoxin immune-affinity array provides a sensitive, rapid, accurate, stable, and convenient platform for detection of multiple mycotoxins in agro-foods. Graphical abstract Paper-based immune-affinity monolithic array. DON deoxynivalenol, HT-2 HT-2 toxin, T-2 T-2 toxin, PEGDA polyethylene glycol diacrylate, ZEN zearalenone