Application of Serum Free Medium Cultured Vero Cells for the Production of Recombinant Oncolytic Herpes Simplex Virus 2

The oncolytic virus, especially the recombinant oncolytic herpes simplex virus 2 (oHSV2), has attracted much attention because of its promising cancer treatment effect. To eliminate animal-derived substances from serum supplemented culture medium, which is an adverse factor for the safety of biologi...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2021-03, Vol.714 (3), p.32001
Main Authors: Fang, Zongyao, Wang, Leilan, Zou, Zongxing, Zhang, Siqi, Zhao, Yin, Hu, Sheng, Liu, Binlei
Format: Article
Language:English
Subjects:
Cell culture
Herpes simplex
Herpes viruses
Oncolysis
Product safety
Vaccines
Vero cells
Viruses
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Summary:The oncolytic virus, especially the recombinant oncolytic herpes simplex virus 2 (oHSV2), has attracted much attention because of its promising cancer treatment effect. To eliminate animal-derived substances from serum supplemented culture medium, which is an adverse factor for the safety of biological products, this study selected three typical serum free media (SFM) on the market, and successfully obtained three SFM cultured Vero cell lines which could be expanded stably. This work provided a reference for the application of SFM to the production of other biological products or vaccine.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/714/3/032001