The Preservation Epididymis Spermatozoa of Buffalo into Talp Diluent with Addition of Serum Level

• Published in: IOP conference series. Earth and environmental science 2021-03, Vol.709 (1), p.12028
• Main Authors: Harissatria, Surtina, Dara, Jaswandi, Hendri, Astuti, T, Afrini, D, Sari, R M, Hendri, J, Asri, A
• Format: Article
• Language: English
• Subjects: Abnormalities; Buffalo; Cryopreservation; Epididymis; Glycerol; Low temperature; Motility; Sperm; Spermatozoa; Yolk
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/709/1/012028

The purpose of this study is to determine the quality of epididymis spermatozoa of buffalo after preservation with TALP media by adding serum with different level. The method used in this study is the experimental method and the design used is a randomized block design (RBD) with 3 treatment by adding bovine serum (0%, 5%, and 10%) and there are 6 replications as a group (repetition in the form of days). The result showed that the addition of serum into TALP diluent had a significant effect (P>0, 05) on the percentage of the motility of cauda epididymis spermatozoa of swamp buffalo after preservation which was 73, 33 in P2 10% serum and the viability is 79,167 ± 2,04 on P2 10%. This can be used as an indicator that the serum is effective in protecting spermatozoa from damages during the cryopreservation process, so by that, it would improve the quality of cauda epididymal spermatozoa of swamp buffalo. While the percentages of abnormality of cauda epididymis spermatozoa of swamp buffalo after preservation did not have a significant effect (P>0, 05). Based on the result of this study it can be concluded that the addition of 5% serum and 10% addition of egg yolk and 5% addition of glycerol will improve the quality of motility and viability and had no adverse effect on abnormalities after the preservation process at low temperature.