A putative 2OG-Fe(II) oxygenase’s response to gibberellin deficiency is related to the internodal growth of columnar apples

Columnar apples are a labor saving and productive tree form that has long been of interest to apple breeders and producers. MdCo31 , which encodes a putative 2OG-Fe(II) oxygenase, is a potential candidate gene involved in controlling the internode length of columnar growth of apple. In this study, a...

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Bibliographic Details
Published in:Acta physiologiae plantarum 2021, Vol.43 (5)
Main Authors: Wang, Limin, Yu, Boyang, Zhao, Yanan, Li, Yongzhou, Guo, Jing, Zhu, YuanDi
Format: Article
Language:English
Subjects:
Agriculture
Apples
Biomedical and Life Sciences
Chlorophyll
Cytoplasm
Flowering
Fruits
Fusion protein
Germination
Gibberellins
Iron
Life Sciences
Original Article
Overexpression
Oxygenase
Phenotypes
Plant Anatomy/Development
Plant Biochemistry
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plants (botany)
Seed germination
Tobacco
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Summary:Columnar apples are a labor saving and productive tree form that has long been of interest to apple breeders and producers. MdCo31 , which encodes a putative 2OG-Fe(II) oxygenase, is a potential candidate gene involved in controlling the internode length of columnar growth of apple. In this study, a putative regulation in internode length was conducted by exogenous application of GA 3 and paclobutrazol. The results showed that the MdCo31 -GFP fusion protein was specifically localized in the cytoplasm. Heterologous over-expression of MdCo31 in tobacco produced stunted phenotypes with high chlorophyll content in leaves, and delayed the timing of seed germination and flowering. The over-expression tobacco plants were more sensitive to exogenous application of GA 3 but insensitive to paclobutrazol, and their growth correlated to lower concentrations of endogenous GA 1 and lower expression levels of the gibberellin-regulated family gene NtGASA6 . The higher the MdCo31 expression levels were identified in more dwarf transformants with the shorter internodes. This research proposed that the decrease in endogenous GA 1 , resulting from high MdCo31 expression, induced dwarf phenotypes and shorted internodes, which indicated that MdCo31 is responded to gibberellins’ deficiency conferring the internodal growth of columnar apples.
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-021-03243-z