Evaluation parameters of in vitro morphogenesis in the process of replication of potato starting material

• Published in: IOP conference series. Earth and environmental science 2021-03, Vol.677 (4), p.42030
• Main Authors: Oves, E, Anikina, I, Gaitova, N, Adamzhanova, Zh, Kaynidenov, N
• Format: Article
• Language: English
• Subjects: Micropropagation; Morphogenesis; Morphology; Plant tissues; Potatoes; Regeneration; Replication; Tissue culture
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/677/4/042030

In the process of replicating and growing the necessary volumes of in vitro material for original seed production, the period of formation of morphological structures and the achievement of microplants of standard characteristics is important. The existing method of regeneration assessment by the quantity of days from the moment of placement of micro stalks into a new nutrient medium does not allow a planned approach to the compilation and implementation of the clonal micropropagation program. The article proposes new methodological approaches to assessing in vitro potato material by the main phases of plant development in tissue culture: intensive growth (formation of 2-3 internodes), slow growth (4-6 internodes), and natural dying-off. The analysis of regenerative ability allows us to note that the effectiveness of the formation of morphological structures did not depend on the ripeness group of the studied variety samples. Presented results indicate the differentiated approach of varieties to the formation of morphological structures in the process of replication.