In Vitro and in Vivo Measurements of ROS Scavenging Activity and White Blood Cells Activity by Chemiluminescence of a New Selena-Diazole Derivative Compare to Dipyrone Activity

Reactive oxygen species ROS scavenging activity of whole Blood and White Blood Cells were measured. The estimation depends on the principle of luminol oxidation by ROS produced during phagocytosis. The study aimed to evaluate ROS production of Whole Blood and WBCs activity. CL measured using Luminol...

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Bibliographic Details
Published in:Journal of physics. Conference series 2021-03, Vol.1818 (1), p.12060
Main Authors: Neamah, Nadheerah Falih, Naaeem Khudair, Abdul-Razzak, Al-Jadaan, Shaker A. N.
Format: Article
Language:English
Subjects:
Antioxidants
Chemical activity
Chemiluminescence
Leukocytes
Oxidation
Physics
Scavenging
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Summary:Reactive oxygen species ROS scavenging activity of whole Blood and White Blood Cells were measured. The estimation depends on the principle of luminol oxidation by ROS produced during phagocytosis. The study aimed to evaluate ROS production of Whole Blood and WBCs activity. CL measured using Luminol-dependent CL reader, and the recording system is Scaler Time ST7. The female rats in four groups received T and/or Di, or received only 2mL of DW for 30 days. CL Invitro antioxidant activity the result exhibited T&Di group was significant reduction in CL. Invivo CL of T group CL activity of whole blood was significant elevated CL than all groups. While T&Di group CL activity was reduced essentially than T, Di group; Functional WBC/100cells invitro CL activity of T&Di decreased significantly than DW CL while the Di, and T groups had no important changed than DW group WBCs CL activity. The results of WBCs invivo CL were T&Di activity was significantly higher than DW activity. Also, in Di the CL activity of WBCs significantly elevated than DW. While T the WBC activity showed non-significant alteration with DW. It was concluded that T compound had a potent ROS scavenging activity with reduced WBCs activity.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/1818/1/012060