In vitro contractile responses of human detrusor smooth muscle to oxytocin: does it really have effect?

The aim of this study was to investigate the contractile effects of oxytocin (OT) in human detrusor muscle in in vitro conditions. Human detrusor muscle samples were obtained from seven patients that undergone radical cystectomy. Four female Wistar rats' uterine samples were used as control. Co...

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Bibliographic Details
Published in:The aging male 2020-12, Vol.23 (5), p.1141-1145
Main Authors: Tarhan, Fatih, Çağlayan Duman, Nesrin, Özkula, Songül, Karaalp, Atila, Cangüven, Önder
Format: Article
Language:English
Subjects:
Aging
Animals
Atosiban
Bladder
Carbachol - pharmacology
Female
Hormones
human
Humans
In Vitro
Muscle Contraction
Muscle, Smooth
Muscular system
oxytocin
Oxytocin - pharmacology
Rats
Rats, Wistar
Rodents
urinary bladder
Urological surgery
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Summary:The aim of this study was to investigate the contractile effects of oxytocin (OT) in human detrusor muscle in in vitro conditions. Human detrusor muscle samples were obtained from seven patients that undergone radical cystectomy. Four female Wistar rats' uterine samples were used as control. Contractile responses were tested of carbachol in organ bath. Cumulative concentration response curves were constructed to OT and then the strips were incubated with atosiban (OT antagonist) and a second concentration response curve to OT were constructed. Carbachol, contracted all human strips for the functionality test whereas OT in any concentrations did not produce significant contraction on all human strips. In only one bladder strip and in a very high concentration slight contraction was recorded. Moreover no contractile response was recorded in any OT concentrations in the presence of atosiban. The rat uterine strips responded to OT in a dose dependent manner. Atosiban, the OT receptor antagonist diminished totally those contractile responses. It is been demonstrated here that there is no contractile response to OT in human detrusor muscle. These findings should be supported by further investigations determining the presence of the OT receptor in human detrusor.
ISSN:1368-5538
1473-0790
DOI:10.1080/13685538.2020.1711722