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New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection
New two-component pyrene probes based on oligo(2'- O -methylribonucleotides) for microRNA detection have been designed. They contain the Py A-modified adenine cluster (pentaadenosine fragment that contains 8-(1-ethynylpyrene)-deoxyriboadenosine in the center) and can form a three-way junction (...
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Published in: | Russian journal of bioorganic chemistry 2021-03, Vol.47 (2), p.432-440 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | New two-component pyrene probes based on oligo(2'-
O
-methylribonucleotides) for microRNA detection have been designed. They contain the
Py
A-modified adenine cluster (pentaadenosine fragment that contains 8-(1-ethynylpyrene)-deoxyriboadenosine in the center) and can form a three-way junction (3WJ) structure with a target RNA. We have chosen microRNA let-7a-3p as the RNA target because of the correlation of its concentration in cells with the appearance and progression of cancer. We have compared the thermal stability and fluorescence properties of the two-component probes based on oligo(2'-
O
-methylribonucleotides) that contain either deoxyriboadenosine (dAdA
Py
AdAdA) or the (2'-
O
-methylribo)adenosine (A
m
A
m Py
AA
m
A
m
) cluster with those of (dAdA
Py
AdAdA)-containing oligodeoxyribonucleotide. The changes in the fluorescence spectra of two-component probes after hybridization with the RNA target have been demonstrated. These probes can be used for designing a new microRNA detection system. |
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ISSN: | 1068-1620 1608-330X |
DOI: | 10.1134/S1068162021020230 |