Neurogenic Potential of Implanted Neurospheres Is Regulated by Optogenetic Stimulation of Hippocampal Astrocytes Ex Vivo

The protocol of optogenetic ChR2-mediated activation of astrocytes was used in a model of artificial neurogenic niche, neurospheres implanted into ex vivo organotypic cultures of mouse hippocampus. The electrophysiological characteristics of the hippocampus and expression of molecules involved in th...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2021-04, Vol.170 (6), p.693-698
Main Authors: Morgun, A. V., Osipova, E. D., Boitsova, E. B., Shuvaev, A. N., Malinovskaya, N. A., Mosiagina, A. I., Salmina, A. B.
Format: Article
Language:English
Subjects:
Astrocytes
Bioavailability
Biomedical and Life Sciences
Biomedicine
Brain slice preparation
CD11b antigen
CD18 antigen
CD38 antigen
Cell Biology
Complement component C3b
Connexin 43
Glial fibrillary acidic protein
Hippocampal plasticity
Hippocampus
Internal Medicine
Laboratory Medicine
Microglia
NAD
Neurogenesis
Neurons
Neurospheres
Pathology
Photoactivation
Physiology
Stem cells
Synaptic plasticity
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Summary:The protocol of optogenetic ChR2-mediated activation of astrocytes was used in a model of artificial neurogenic niche, neurospheres implanted into ex vivo organotypic cultures of mouse hippocampus. The electrophysiological characteristics of the hippocampus and expression of molecules involved in the mechanisms of activation of astrocytes and microglia (GFAP, CD38, C3/C3b, Cx43, CD11b, and CD18) were evaluated. Photoactivation of astrocytes led to activation of neurogenesis and changes in the expression of molecules (Cx43 and CD38) that determine bioavailability of NAD + to ensure proliferative activity of cells in the neurogenic niche. Implantation of neurospheres into organotypic slices of the hippocampus caused an increase in C3/C3b expression and suppression of the synaptic plasticity of hippocampal neurons.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-021-05135-1