Lysates of Metschnikowia yeast with higher content of hydroxyproline

The chemical characteristics of lysates obtained from yeasts belonging to Metschnikowia spp. were determined. Cell lysis was induced using saponin from Q. saponaria or 5% NaCl. The process was conducted at 50 °C for 24 for 48 h. The enzymatic profiles of the resulting lysates were analyzed. The mann...

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Bibliographic Details
Published in:Bioresources 2020-05, Vol.15 (2), p.3228-3236
Main Authors: Pawlikowska, Ewelina, Szymańska, Monika, Berłowska, Joanna, Kręgiel, Dorota
Format: Article
Language:English
Subjects:
Amino acids
Carbohydrates
Cell cycle
Enzymes
Fourier transforms
Glucose
Hydroxyproline
Industrial strains
Lysates
Lysis
Mannose
Metschnikowia
Nitrogen
Phosphatase
Proteins
Saponins
Sodium chloride
Yeast
Yeasts
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Summary:The chemical characteristics of lysates obtained from yeasts belonging to Metschnikowia spp. were determined. Cell lysis was induced using saponin from Q. saponaria or 5% NaCl. The process was conducted at 50 °C for 24 for 48 h. The enzymatic profiles of the resulting lysates were analyzed. The mannose and glucose contents were also investigated, as well as the concentrations of proteins, free amino nitrogen (FAN), and free amino acids. The results were compared to the characteristics of lysates from conventional industrial strains of Saccharomyces spp. obtained under analogous conditions. The Metschnikowia lysates showed different chemical profiles and the pool of individual amino acids was generally smaller. However, the content of hydroxyproline HPro was 4 to 5 times higher. The results of this study show that yeast lysates are an attractive supplement for numerous applications.
ISSN:1930-2126
1930-2126
DOI:10.15376/biores.15.2.3228-3236