Dose- and time-dependent systemic adverse reactions of sodium carboxy methyl cellulose after intraperitoneal application in rats

• Published in: Journal of toxicological sciences 2021, Vol.46(5), pp.223-234
• Main Authors: Fan, Chunguang, Hou, Li, Che, Guoxi, Shi, Yanping, Liu, Xiangdong, Sun, Likui, Jia, Wensi, Zhu, Fuyu, Zhao, Zenglin, Xu, Ming, Gai, Xiaoxiao, Liu, Chenghu
• Format: Article
• Language: English
• Subjects: Absorbable biomaterials; Adrenal Glands - drug effects; Adrenal Glands - pathology; Adverse effects; Animals; Biocompatibility; Biomaterials; Biomedical materials; Carboxymethyl cellulose; Carboxymethylcellulose Sodium - toxicity; Cellulose; Dosage; Dose- and time-dependent effects; Dose-Response Relationship, Drug; Female; Hemostasis; Hemostatics; Injection; Injections, Intraperitoneal; Intraperitoneal application; Kidney - drug effects; Kidney - pathology; Liver - drug effects; Liver - pathology; Male; Medical equipment; Methylcellulose; Pancreas - drug effects; Pancreas - pathology; Rats; Rats, Sprague-Dawley; Rodents; Side effects; Sodium; Sodium carboxy methyl cellulose; Spleen - drug effects; Spleen - pathology; Systemic toxicity study; Thymus Gland - drug effects; Thymus Gland - pathology; Time dependence; Toxicity; Uterus - drug effects; Uterus - pathology
• ISSN: 0388-1350; 1880-3989
• DOI: 10.2131/jts.46.223

Sodium carboxy methyl cellulose (SCMC) is an important absorbable biomaterial for anti-adhesion and hemostasis medical devices used in the abdominal cavity. However, the systemic toxicity of SCMC following intraperitoneal route has not been revealed sufficiently. Three SCMC solutions with gradient concentrations were intraperitoneally injected into 3 groups of rats with the doses of 50 mg/kg, 320 mg/kg and 2000 mg/kg respectively all at once to observe the dose-dependence of systemic reactions of SCMC and 10 rats (5 rats per sex) of each group were sacrificed 3 days, 7 days, 28 days and 90 days after injection to evaluate the time-dependence of the reactions. A range of adverse effects were shown in rats of the high-dose group which were found varied with time extending and virtually disappeared 90 days after injection. Slight reactions were observed in the medium-dose group while negligible effects were found in the low-dose group. The intraperitoneal application of SCMC can induce reversible systemic adverse effects to rats at the dose higher than 320 mg/kg and it is essential to take both dose- and time-dependent effects into account while designing a systemic toxicity study for absorbable biomaterials.