Determination of spatial mercury concentration by laser ablation-inductively coupled plasma mass spectrometry

Laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS) is capable of metal imaging by acquiring local spatial information. However, the preparation of an appropriate standard for quantitative analysis is difficult because the matrices between the standard and the sample should match...

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Bibliographic Details
Published in:Journal of toxicological sciences 2021, Vol.46(5), pp.193-198
Main Authors: Iwase, Makiko, Tanaka, Yu-ki, Suzuki, Noriyuki, Ogra, Yasumitsu
Format: Article
Language:English
Subjects:
Ablation
Animal tissues
Animals
Atomic absorption analysis
Atomic absorption spectrophotometry
Cold vaper atomic absorption spectrophotometry
Emission spectroscopy
Homogeneity
ICP-MS
Image acquisition
Inductively coupled plasma mass spectrometry
Kidney
Kidney - chemistry
Kidney - metabolism
Kidneys
Laser ablation
Lasers
Linearity
Liver - chemistry
Liver - metabolism
Male
Mass spectrometry
Mass spectroscopy
Mercury
Mercury (metal)
Mercury - analysis
Mercury - metabolism
Metal concentrations
Methylmercury
Methylmercury Compounds - pharmacokinetics
Quantitative analysis
Rats
Rats, Wistar
Scientific imaging
Spatial data
Spectral analysis
Spectrophotometry
Spectrum Analysis - methods
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Summary:Laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS) is capable of metal imaging by acquiring local spatial information. However, the preparation of an appropriate standard for quantitative analysis is difficult because the matrices between the standard and the sample should match, and homogeneity of metal concentration in the standard is required. Hence, the aim of this study was to establish a highly quantitative mercury imaging method that utilizes LA-ICP-MS and an appropriate mercury standard consisting of rat tissue. Our standard showed homogeneous mercury concentration and good linearity between concentration and signal intensity, and met the qualifications for quantitative imaging by LA-ICP-MS. Mercury concentration in MeHg-exposed rat kidneys obtained by LA-ICP-MS measurement of the standard (7.84 ± 0.57 µg/g) was comparable to that obtained by cold vapor atomic absorption spectrophotometry (AAS, 7.27 ± 0.46 µg/g). The results indicate that LA-ICP-MS enabled quantitative imaging with the appropriate standard.
ISSN:0388-1350
1880-3989
DOI:10.2131/jts.46.193