Knock-down of transcription factor skinhead-1 exacerbates arsenite-induced oxidative damage in Caenorhabditis elegans

Transcription factor, skinhead-1 ( skn-1 ) has been demonstrated to play central roles in regulation of oxidative damage. Arsenite is an oxidative damage inducer in the environment. However, the role of skn-1 in arsenite-induced oxidative damage remains unclear. Thus, in this study, by using RNAi fe...

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Bibliographic Details
Published in:Biometals 2021-06, Vol.34 (3), p.675-686
Main Authors: Mao, Yijie, Yao, Ling, Jiang, Xuejun, Sumayyah, Golamaully, Zou, Zhen, Yi, Qiying, Chen, Chengzhi
Format: Article
Language:English
Subjects:
Animals
Arsenite
Arsenites - administration & dosage
Arsenites - toxicity
Behavior, Animal - drug effects
Biochemistry
Biomedical and Life Sciences
Caenorhabditis elegans - drug effects
Caenorhabditis elegans - metabolism
Cell Biology
Damage
Life Sciences
Medicine/Public Health
Microbiology
Nematodes
Oxidation
Oxidative Stress - drug effects
Pharmacology/Toxicology
Plant Physiology
RNA-mediated interference
Survival
Transcription factors
Transcription Factors - antagonists & inhibitors
Transcription Factors - metabolism
Worms
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Summary:Transcription factor, skinhead-1 ( skn-1 ) has been demonstrated to play central roles in regulation of oxidative damage. Arsenite is an oxidative damage inducer in the environment. However, the role of skn-1 in arsenite-induced oxidative damage remains unclear. Thus, in this study, by using RNAi feeding, different toxic responses of wild-type and skn-1 knockdown nematodes to arsenite were evaluated. Our results demonstrated that arsenite did not show any significant impacts on locomotory behaviors, but skn-1 knock-down worms were much more sensitive to arsenite treatment, manifested by an aggravated reduction of survival rate than that of wild-type nematodes. In arsenite-treated worms, down-regulation of skn-1 significantly exacerbated the arsenite-induced changed expressions of oxidative damage-related genes , xbp-1 , apl-1 and trxr-2 , but these regulated effects of skn-1 were not observed on spr-4 and sel-12 expressions under arsenite treatment. These findings together suggest that skn-1 may play a vital role in protection of C. elegans from arsenite-induced oxidative damage.
ISSN:0966-0844
1572-8773
DOI:10.1007/s10534-021-00303-2