Changes induced by lead in root system architecture of Arabidopsis seedlings are mediated by PDR2-LPR1/2 phosphate dependent way

• Published in: Biometals 2021-06, Vol.34 (3), p.603-620
• Main Authors: Ortiz-Luevano, Ricardo, López-Bucio, José, Martínez-Trujillo, Miguel, Sánchez-Calderón, Lenin
• Format: Article
• Language: English
• Subjects: Acidification; Adenosine Triphosphatases - metabolism; Arabidopsis - drug effects; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - metabolism; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cell division; Changing environments; Computer architecture; Crop production; Dose-Response Relationship, Drug; Environmental changes; Environmental effects; Environmental stress; Homeostasis; Lead; Lead - chemistry; Lead - metabolism; Lead - pharmacology; Life Sciences; Medicine/Public Health; Metabolism; Metals; Microbiology; Morphology; Mutants; Oxidoreductases - metabolism; Pharmacology/Toxicology; Phosphates - metabolism; Physiological responses; Physiology; Plant Physiology; Plant Roots - drug effects; Plant Roots - growth & development; Plant Roots - metabolism; Pollutants; Roots; Seedlings; Seedlings - drug effects; Seedlings - growth & development; Seedlings - metabolism; Sessile species
• ISSN: 0966-0844; 1572-8773
• DOI: 10.1007/s10534-021-00299-9

As sessile organisms, plants respond to changing environments modulating their genetic expression, metabolism and postembryonic developmental program (PDP) to adapt. Among environmental stressor, lead (Pb) is one of the most hazardous pollutants which limits crop productivity. Here, we describe in detail the effects of a wide range of concentrations of Pb on growth and development and a possible convergence with phosphate (Pi) starvation response. We found that the response to Pb presents a biphasic curve dose response in biomass accumulation: below 400 µM show a stimulatory effect meanwhile at Pb doses up to 600 µM effects are inhibitory. We found that +Pb (800 µM) modifies root system architecture (RSA) and induces acidification media, according to in silico ion interaction, in the growing medium Pb and Pi coprecipitate and plants grow in both Pi deficiency and Pb stress at the same time, however in spite of seedlings are under Pi starvation AtPT2 expression are Pb downregulated indicating that in addition to Pi starvation stress, Pb regulates physiological responses in root system. Using the mutants stop1, lpr1/2 and lpi3, which are affected in Pi starvation response, we found that changes in RSA by +Pb is genetically regulated and there are shared pathways with Pi starvation response mediated by PDR2-LPR1/2 and LPI3 pathways since lpr1/2 and lpi3 mutants are insensitive to +Pb and Pi starvation. Taking together, these results indicate that similar changes in RSA induced by independent environmental stimuli +Pb and Pi starvation are due to similar mediated response by PDR2-LPR1/2 pathway.