Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system

Aims To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo‐inducible λcI857‐pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis gene E during the production of bacterial ghosts (BGs). Methods and results The p...

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Bibliographic Details
Published in:Journal of applied microbiology 2021-06, Vol.130 (6), p.2008-2017
Main Authors: Fu, L.X., Gong, J.S., Gao, B., Ji, D.J., Han, X.G., Zeng, L.B.
Format: Article
Language:English
Subjects:
Bacteria
bacterial ghosts
cI857 repressor
E coli
Escherichia coli
Gene expression
Ghosts
Lysis
lysis gene E
Mutagenesis
Mutants
Mutation
Phenotypes
promoter pL
Random mutagenesis
Salmonella
Salmonella enteritidis
Site-directed mutagenesis
Stability analysis
Thermal stability
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Summary:Aims To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo‐inducible λcI857‐pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis gene E during the production of bacterial ghosts (BGs). Methods and results The promoter pL mutant was identified by random mutagenesis and site‐directed mutagenesis. The results showed that a T → 35C mutation in the pL promoter was responsible for the phenotype alteration. Under the same induction conditions, the lysis rates of the modified lytic system on Escherichia coli and Salmonella enteritidis were significantly lower than that of the control, while the lysis rates of Escherichia coli with the thermo‐inducible lytic system were significantly higher than that of S. enteritidis with the corresponding plasmid (P 
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.14690