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Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system
Aims To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo‐inducible λcI857‐pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis gene E during the production of bacterial ghosts (BGs). Methods and results The p...
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| Published in: | Journal of applied microbiology 2021-06, Vol.130 (6), p.2008-2017 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c3530-db066585e7041b2db5da99d3361d3ef5b77c17dd0e62082076859a6233edb483 |
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| cites | cdi_FETCH-LOGICAL-c3530-db066585e7041b2db5da99d3361d3ef5b77c17dd0e62082076859a6233edb483 |
| container_end_page | 2017 |
| container_issue | 6 |
| container_start_page | 2008 |
| container_title | Journal of applied microbiology |
| container_volume | 130 |
| creator | Fu, L.X. Gong, J.S. Gao, B. Ji, D.J. Han, X.G. Zeng, L.B. |
| description | Aims
To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo‐inducible λcI857‐pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis gene E during the production of bacterial ghosts (BGs).
Methods and results
The promoter pL mutant was identified by random mutagenesis and site‐directed mutagenesis. The results showed that a T → 35C mutation in the pL promoter was responsible for the phenotype alteration. Under the same induction conditions, the lysis rates of the modified lytic system on Escherichia coli and Salmonella enteritidis were significantly lower than that of the control, while the lysis rates of Escherichia coli with the thermo‐inducible lytic system were significantly higher than that of S. enteritidis with the corresponding plasmid (P |
| doi_str_mv | 10.1111/jam.14690 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2524661752</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2524661752</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3530-db066585e7041b2db5da99d3361d3ef5b77c17dd0e62082076859a6233edb483</originalsourceid><addsrcrecordid>eNp1kE1OwzAQhS0EoqWw4ALIEisWaf0T2-myqgoUFbHpPkriKaRK4mInguw4AvfhDhyCk-A2LTtGGs1o5tOb0UPokpIh9TFaJ-WQhnJMjlCfcikCJhU73vVhIIhiPXTm3JoQyomQp6jHGRdRxEQf2ampamuKAjSG940F53JTYbPCRetyh5-hAjzDaYsTXDZ1UtXbXf0CeGNNaWqweLM4jHza0vx8fOaVbrI8LQB_f2XzSCg_85hrXQ3lOTpZJYWDi30doOXtbDm9DxZPd_PpZBFkXHAS6JRIKSIBioQ0ZToVOhmPNeeSag4rkSqVUaU1AclIxIiSkRgnknEOOg0jPkDXnax_9LUBV8dr09jKX4yZYKGUVAnmqZuOyqxxzsIq3ti8TGwbUxJvzY29ufHOXM9e7RWbtAT9Rx7c9MCoA97yAtr_leKHyWMn-Qs8boUd</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2524661752</pqid></control><display><type>article</type><title>Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system</title><source>Alma/SFX Local Collection</source><creator>Fu, L.X. ; Gong, J.S. ; Gao, B. ; Ji, D.J. ; Han, X.G. ; Zeng, L.B.</creator><creatorcontrib>Fu, L.X. ; Gong, J.S. ; Gao, B. ; Ji, D.J. ; Han, X.G. ; Zeng, L.B.</creatorcontrib><description>Aims
To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo‐inducible λcI857‐pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis gene E during the production of bacterial ghosts (BGs).
Methods and results
The promoter pL mutant was identified by random mutagenesis and site‐directed mutagenesis. The results showed that a T → 35C mutation in the pL promoter was responsible for the phenotype alteration. Under the same induction conditions, the lysis rates of the modified lytic system on Escherichia coli and Salmonella enteritidis were significantly lower than that of the control, while the lysis rates of Escherichia coli with the thermo‐inducible lytic system were significantly higher than that of S. enteritidis with the corresponding plasmid (P < 0·05).
Conclusions
Increasing the heat stability of the thermo‐inducible lytic systems decreased lysis efficiency during the production of BGs. There exist differences in the lysis efficiency of thermo‐inducible lytic systems between different bacterial strains.
Significance and Impact of the Study
These findings enrich current knowledge about modifications to thermo‐inducible systems and provide a reference for the application of these modified systems for the production of BGs and controlled gene expression in bacteria.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14690</identifier><identifier>PMID: 32358825</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Bacteria ; bacterial ghosts ; cI857 repressor ; E coli ; Escherichia coli ; Gene expression ; Ghosts ; Lysis ; lysis gene E ; Mutagenesis ; Mutants ; Mutation ; Phenotypes ; promoter pL ; Random mutagenesis ; Salmonella ; Salmonella enteritidis ; Site-directed mutagenesis ; Stability analysis ; Thermal stability</subject><ispartof>Journal of applied microbiology, 2021-06, Vol.130 (6), p.2008-2017</ispartof><rights>2020 The Society for Applied Microbiology</rights><rights>2020 The Society for Applied Microbiology.</rights><rights>Copyright © 2021 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3530-db066585e7041b2db5da99d3361d3ef5b77c17dd0e62082076859a6233edb483</citedby><cites>FETCH-LOGICAL-c3530-db066585e7041b2db5da99d3361d3ef5b77c17dd0e62082076859a6233edb483</cites><orcidid>0000-0001-5625-637X ; 0000-0002-4906-1053</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32358825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, L.X.</creatorcontrib><creatorcontrib>Gong, J.S.</creatorcontrib><creatorcontrib>Gao, B.</creatorcontrib><creatorcontrib>Ji, D.J.</creatorcontrib><creatorcontrib>Han, X.G.</creatorcontrib><creatorcontrib>Zeng, L.B.</creatorcontrib><title>Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo‐inducible λcI857‐pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis gene E during the production of bacterial ghosts (BGs).
Methods and results
The promoter pL mutant was identified by random mutagenesis and site‐directed mutagenesis. The results showed that a T → 35C mutation in the pL promoter was responsible for the phenotype alteration. Under the same induction conditions, the lysis rates of the modified lytic system on Escherichia coli and Salmonella enteritidis were significantly lower than that of the control, while the lysis rates of Escherichia coli with the thermo‐inducible lytic system were significantly higher than that of S. enteritidis with the corresponding plasmid (P < 0·05).
Conclusions
Increasing the heat stability of the thermo‐inducible lytic systems decreased lysis efficiency during the production of BGs. There exist differences in the lysis efficiency of thermo‐inducible lytic systems between different bacterial strains.
Significance and Impact of the Study
These findings enrich current knowledge about modifications to thermo‐inducible systems and provide a reference for the application of these modified systems for the production of BGs and controlled gene expression in bacteria.</description><subject>Bacteria</subject><subject>bacterial ghosts</subject><subject>cI857 repressor</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Gene expression</subject><subject>Ghosts</subject><subject>Lysis</subject><subject>lysis gene E</subject><subject>Mutagenesis</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Phenotypes</subject><subject>promoter pL</subject><subject>Random mutagenesis</subject><subject>Salmonella</subject><subject>Salmonella enteritidis</subject><subject>Site-directed mutagenesis</subject><subject>Stability analysis</subject><subject>Thermal stability</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kE1OwzAQhS0EoqWw4ALIEisWaf0T2-myqgoUFbHpPkriKaRK4mInguw4AvfhDhyCk-A2LTtGGs1o5tOb0UPokpIh9TFaJ-WQhnJMjlCfcikCJhU73vVhIIhiPXTm3JoQyomQp6jHGRdRxEQf2ampamuKAjSG940F53JTYbPCRetyh5-hAjzDaYsTXDZ1UtXbXf0CeGNNaWqweLM4jHza0vx8fOaVbrI8LQB_f2XzSCg_85hrXQ3lOTpZJYWDi30doOXtbDm9DxZPd_PpZBFkXHAS6JRIKSIBioQ0ZToVOhmPNeeSag4rkSqVUaU1AclIxIiSkRgnknEOOg0jPkDXnax_9LUBV8dr09jKX4yZYKGUVAnmqZuOyqxxzsIq3ti8TGwbUxJvzY29ufHOXM9e7RWbtAT9Rx7c9MCoA97yAtr_leKHyWMn-Qs8boUd</recordid><startdate>202106</startdate><enddate>202106</enddate><creator>Fu, L.X.</creator><creator>Gong, J.S.</creator><creator>Gao, B.</creator><creator>Ji, D.J.</creator><creator>Han, X.G.</creator><creator>Zeng, L.B.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0001-5625-637X</orcidid><orcidid>https://orcid.org/0000-0002-4906-1053</orcidid></search><sort><creationdate>202106</creationdate><title>Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system</title><author>Fu, L.X. ; Gong, J.S. ; Gao, B. ; Ji, D.J. ; Han, X.G. ; Zeng, L.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3530-db066585e7041b2db5da99d3361d3ef5b77c17dd0e62082076859a6233edb483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bacteria</topic><topic>bacterial ghosts</topic><topic>cI857 repressor</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Gene expression</topic><topic>Ghosts</topic><topic>Lysis</topic><topic>lysis gene E</topic><topic>Mutagenesis</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Phenotypes</topic><topic>promoter pL</topic><topic>Random mutagenesis</topic><topic>Salmonella</topic><topic>Salmonella enteritidis</topic><topic>Site-directed mutagenesis</topic><topic>Stability analysis</topic><topic>Thermal stability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, L.X.</creatorcontrib><creatorcontrib>Gong, J.S.</creatorcontrib><creatorcontrib>Gao, B.</creatorcontrib><creatorcontrib>Ji, D.J.</creatorcontrib><creatorcontrib>Han, X.G.</creatorcontrib><creatorcontrib>Zeng, L.B.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, L.X.</au><au>Gong, J.S.</au><au>Gao, B.</au><au>Ji, D.J.</au><au>Han, X.G.</au><au>Zeng, L.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2021-06</date><risdate>2021</risdate><volume>130</volume><issue>6</issue><spage>2008</spage><epage>2017</epage><pages>2008-2017</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo‐inducible λcI857‐pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis gene E during the production of bacterial ghosts (BGs).
Methods and results
The promoter pL mutant was identified by random mutagenesis and site‐directed mutagenesis. The results showed that a T → 35C mutation in the pL promoter was responsible for the phenotype alteration. Under the same induction conditions, the lysis rates of the modified lytic system on Escherichia coli and Salmonella enteritidis were significantly lower than that of the control, while the lysis rates of Escherichia coli with the thermo‐inducible lytic system were significantly higher than that of S. enteritidis with the corresponding plasmid (P < 0·05).
Conclusions
Increasing the heat stability of the thermo‐inducible lytic systems decreased lysis efficiency during the production of BGs. There exist differences in the lysis efficiency of thermo‐inducible lytic systems between different bacterial strains.
Significance and Impact of the Study
These findings enrich current knowledge about modifications to thermo‐inducible systems and provide a reference for the application of these modified systems for the production of BGs and controlled gene expression in bacteria.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>32358825</pmid><doi>10.1111/jam.14690</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5625-637X</orcidid><orcidid>https://orcid.org/0000-0002-4906-1053</orcidid></addata></record> |
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| source | Alma/SFX Local Collection |
| subjects | Bacteria bacterial ghosts cI857 repressor E coli Escherichia coli Gene expression Ghosts Lysis lysis gene E Mutagenesis Mutants Mutation Phenotypes promoter pL Random mutagenesis Salmonella Salmonella enteritidis Site-directed mutagenesis Stability analysis Thermal stability |
| title | Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system |
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