Potentiometric enzyme biosensor for rapid determination of formaldehyde based on succinimide-functionalized polyacrylate ion-selective membrane

• Published in: Measurement : journal of the International Measurement Confederation 2021-04, Vol.175, p.109112, Article 109112
• Main Authors: Nurlely, Ahmad, Musa, Yook Heng, Lee, Ling Tan, Ling
• Format: Article
• Language: English
• Subjects: Acrylic resins; Alcohol oxidase; Batteries; Biosensors; Electrodes; Enzymes; Ethanol; Formaldehyde; Membranes; Nanoparticles; Polyacrylate; Potentiometric biosensor; Response time; Screen-printed electrode; Sensitivity; Silver chloride; Spectrophotometry
• ISSN: 0263-2241; 1873-412X
• DOI: 10.1016/j.measurement.2021.109112

[Display omitted] •pnBA-NAS membrane as both enzyme supporting matrix and pH-sensitive transducer.•Loading of NAS monomer enhanced elasticity and flexibility of the sensing membrane.•Rapid detection via direct formaldehyde accessibility at AOX catalytic film surface.•Potentiometric quantification of formaldehyde by ion transfer reaction at the interface. A membrane-based potentiometric biosensor has been fabricated from poly(n-butyl acrylate-co-N-acryloxysuccinimide as both enzyme supporting matrix and pH-sensitive transducer on the Ag/AgCl screen-printed electrode for rapid and facile determination of formaldehyde. Detection of formaldehyde was based on H+ ion transfer reaction at the electrode–electrolyte interface facilitated with immobilized hydrogen ionophore via ion-ionophore complexation at the interface. The potentiometric enzyme biosensor demonstrated a broad linear range to formaldehyde from 0.5 to 220.0 mM (R2 = 0.9982) with fast response time within 8 s and sensitivity of 59.23 ± 0.85 mV/decade (n = 5). The operational stability of the formaldehyde biosensor with covalently immobilized alcohol oxidase showed > 95% sensitivity throughout the biosensor lifetime experimental period of 80 days. The formaldehyde biosensor based on succinimide-functionalized polyacrylate ion-selective membrane sustained negligible interference of potential interferants, e.g. methanol, ethanol, glucose and glycerol, and its feasibility for accurate assay of formaldehyde in commercial fish samples has been validated with UV–Vis spectrophotometric Nash standard method.