Spatiotemporal distribution of Flavobacterium psychrophilum and ayu Plecoglossus altivelis in rivers revealed by environmental DNA analysis

Outbreaks of bacterial cold-water disease (BCWD), caused by Flavobacterium psychrophilum , are widespread in Japan, especially among ayu Plecoglossus altivelis . There are few investigations of F. psychrophilum in river water, and its seasonal distribution has not been clarified. We aimed to identif...

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Bibliographic Details
Published in:Fisheries science 2021-05, Vol.87 (3), p.321-330
Main Authors: Tenma, Hinako, Tsunekawa, Koki, Fujiyoshi, Riho, Takai, Hajime, Hirose, Masae, Masai, Nanami, Sumi, Kosuke, Takihana, Yuta, Yanagisawa, Sogen, Tsuchida, Kota, Ohara, Kenichi, Jo, Toshiaki, Takagi, Masaki, Ota, Akiko, Iwata, Hiroyoshi, Yaoi, Yuichi, Minamoto, Toshifumi
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Deoxyribonucleic acid
DNA
Dynamics
Environmental DNA
Fish
Fish & Wildlife Biology & Management
Flavobacterium psychrophilum
Food Science
Freshwater & Marine Ecology
Husbandry diseases
Life Sciences
Original Article
Plecoglossus altivelis
River water
Rivers
Seasonal distribution
Spatial distribution
Temperature dependence
Temporal distribution
Water analysis
Water sampling
Water temperature
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Summary:Outbreaks of bacterial cold-water disease (BCWD), caused by Flavobacterium psychrophilum , are widespread in Japan, especially among ayu Plecoglossus altivelis . There are few investigations of F. psychrophilum in river water, and its seasonal distribution has not been clarified. We aimed to identify the spatiotemporal dynamics of F. psychrophilum and ayu to provide information that is useful for establishing a countermeasure for BCWD. Quantitative analysis of environmental DNA (eDNA) was used to clarify the year-round dynamics of ayu and F. psychrophilum . We sampled river water from the Nagara and Ibi rivers in Japan, and conducted monthly water sampling and eDNA quantification. Changes in the eDNA concentration of ayu were consistent with the known life histories of the fish. There was a strong negative correlation between the eDNA concentration of F. psychrophilum and water temperature, suggesting a strong dependence of F. psychrophilum dynamics in the river on water temperature. Furthermore, relatively high eDNA concentrations were recorded for both organisms in early summer and fall, suggesting that ayu is infected with F. psychrophilum during these seasons when experiencing up- and downmigration, respectively.
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-021-01510-z