Design of vesicle prototissues as a model for cellular tissues

Synthesizing biomimetic prototissues with predictable physical properties is a promising tool for the study of cellular tissues, as they would enable to test systematically the role of individual physical mechanisms on complex biological processes. The aim of this study is to design a biomimetic coh...

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Bibliographic Details
Published in:Soft matter 2021-05, Vol.17 (19), p.561-572
Main Authors: Casas-Ferrer, Laura, Brisson, Amaury, Massiera, Gladys, Casanellas, Laura
Format: Article
Language:English
Subjects:
Adhesion
Assembly
Biological activity
Biological Physics
Biomimetics
Biotin
Cell adhesion
Condensed Matter
Deoxyribonucleic acid
DNA
Mechanical properties
Morphology
Physical properties
Physics
Soft Condensed Matter
Streptavidin
Synthesis
Tissues
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Summary:Synthesizing biomimetic prototissues with predictable physical properties is a promising tool for the study of cellular tissues, as they would enable to test systematically the role of individual physical mechanisms on complex biological processes. The aim of this study is to design a biomimetic cohesive tissue with tunable mechanical properties by the controlled assembly of giant unillamelar vesicles (GUV). GUV-GUV specific adhesion is mediated by the inclusion of the streptavidin-biotin pair, or DNA complementary strands. Using a simple assembly protocol, we are capable of synthesizing vesicle prototissues of spheroidal or sheet-like morphologies, with predictable cell-cell adhesion strengths, typical sizes, and degree of compaction. Biomimetic vesicle prototissues have been designed as a model for cellular tissues. Prototissues display spheroidal or sheet like morphologies, and have predictable vesicle-vesicle adhesion strengths, typical sizes, and degree of compaction.
ISSN:1744-683X
1744-6848
DOI:10.1039/d1sm00336d