Coral microbiome changes over the day–night cycle

• Published in: Coral reefs 2021-06, Vol.40 (3), p.921-935
• Main Authors: Caughman, Alicia M., Pratte, Zoe A., Patin, Nastassia V., Stewart, Frank J.
• Format: Article
• Language: English
• Subjects: Archaea; Bacteria; Biomedical and Life Sciences; Colonies; Composition; Coral reefs; Corals; Deoxyribonucleic acid; Diel periodicity; Dinoflagellates; DNA; Ecological function; Environmental conditions; Fluctuations; Freshwater & Marine Ecology; Gene expression; Gene sequencing; Heterogeneity; Hypoxia; Life Sciences; Metabolism; Microbiomes; Night; Oceanography; Pathogens; Periodic variations; Periodicity; Physiology; Porites lutea; rRNA 16S; Seawater; Stability; Stochasticity; Taxonomy; Transcription
• ISSN: 0722-4028; 1432-0975
• DOI: 10.1007/s00338-021-02097-8

Coral-associated microbiomes play important roles in host health and metabolism. It is therefore critical to understand the factors that shape microbiome taxonomic composition and function. Little is known about the stability of coral microbiomes over hourly fluctuations in environmental conditions. For parameters such as temperature and pH, the magnitude of day–night fluctuations can exceed those observed in mean values over seasons, especially on shallow reefs. Such short-term heterogeneity can affect longer term trends, possibly by influencing the extent to which corals acclimate to stress and increase resilience. This study examined diel dynamics of microbiomes (Bacteria and Archaea) in three species of stony coral ( Porites lutea, Porites cylindrica, and Pocillopora damicornis) from a shallow reef in Mo’orea (French Polynesia). We assessed microbiome composition and relative transcriptional activity by analyzing 16S rRNA gene and transcript sequences from six timepoints over 48 h for each species. Both the composition and transcription of microbiome members changed significantly over the diel cycle. Most changes did not exhibit clear diel periodicity but were instead stochastic, a pattern potentially influenced also by microspatial variation within a coral colony. However, a subset of taxa, notably the dinoflagellate-associated bacterium Porticoccus and bacteria of the ubiquitous coral-associated Endozoicomonas genus, exhibited cyclic changes in relative frequency over the 2 d sampling period in the DNA dataset of Porites lutea . These patterns raise the possibility that ecological functions of the microbiome may vary substantially over hourly timescales, notably between day and night. Further studies are needed to compare temporal change in the coral microbiome with that due to other factors, such as spatial distance within and among colonies.