Cycloastragenol inhibits Aβ1-42-induced blood-brain barrier disruption and enhances soluble Aβ efflux in vitro

This study aimed to evaluate the effect Cycloastragenol (CAG), a triterpenoid saponin isolated from the Radix astragali, on Aβ-induced BBB damage. An immortalized endothelial cell line (bEnd.3) was employed to mimic a BBB. The Western blot, TUNEL staining, Flow cytometric analysis and Enzyme-linked...

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Bibliographic Details
Published in:Journal of asian natural products research 2021-06, Vol.23 (6), p.556-569
Main Authors: Huang, Min-Ye, Yu, Gu-Ran
Format: Article
Language:English
Subjects:
1-42
Alzheimer's disease
amyloid-β
Apoptosis
Blood-brain barrier
cycloastragenol
Efflux
Endothelial cells
Enzyme-linked immunosorbent assay
Flow cytometry
Saponins
Trinucleotide repeats
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Summary:This study aimed to evaluate the effect Cycloastragenol (CAG), a triterpenoid saponin isolated from the Radix astragali, on Aβ-induced BBB damage. An immortalized endothelial cell line (bEnd.3) was employed to mimic a BBB. The Western blot, TUNEL staining, Flow cytometric analysis and Enzyme-linked immunosorbent assay were performed. The present results showed that CAG (10, 50, 75 μM) can alleviate oligomer Aβ 1-42 induced bEnd.3 cell apoptosis and increase tight junction scaffold proteins expression. The result also indicated that CAG increased soluble Aβ efflux across BBB via upregulation of the P-gp and downregulation of RAGE expression.
ISSN:1028-6020
1477-2213
DOI:10.1080/10286020.2020.1786372