Characterization and Biotechnological Functional Activities of Exopolysaccharides Produced by Lysinibacillus fusiformis KMNTT-10

In this study, the production and physicochemical characterization of exopolysaccharide (EPS) produced by Lysinibacillus fusiformis KMNTT-10 were investigated. Also, in vitro assays were conducted to investigate the biotechnological functional activities of EPS, including flocculation, emulsificatio...

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Bibliographic Details
Published in:Journal of polymers and the environment 2021-06, Vol.29 (6), p.1742-1751
Main Authors: Mathivanan, Krishnamurthy, Chandirika, Jayaraman Uthaya, Vinothkanna, Annadurai, Govindarajan, R. K., Meng, Delong, Yin, Huaqun
Format: Article
Language:English
Subjects:
Antioxidants
Arabinose
Biotechnology
Chemistry
Chemistry and Materials Science
Emulsification
Environmental Chemistry
Environmental Engineering/Biotechnology
Exopolysaccharides
Flocculation
Free radicals
Functional groups
Galactose
Industrial applications
Industrial Chemistry/Chemical Engineering
Lysinibacillus
Materials Science
Monosaccharides
Morphology
Nitric oxide
Original Paper
Polymer Sciences
Rhamnose
Scavenging
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Summary:In this study, the production and physicochemical characterization of exopolysaccharide (EPS) produced by Lysinibacillus fusiformis KMNTT-10 were investigated. Also, in vitro assays were conducted to investigate the biotechnological functional activities of EPS, including flocculation, emulsification, and antioxidant activity. Results showed that the maximum EPS produced by strain KMNTT-10 was 4.19 g/l after 120 h. The monosaccharides present in EPS were xylose, rhamnose, arabinose, galactose, and glucose in varying combinations. Characterization of EPS by FTIR and XRD analysis revealed the presence of hydroxyl, amino, and carboxyl functional groups and further, the EPS is an amorphous nature. SEM envisaged a highly porous web-like structure with an irregular surface in the EPS morphology. Further, the EPS had good flocculation activity with a maximum of 89.66% and also had potent emulsifying activity towards hydrocarbons with 1% EPS concentration. The EPS showed good antioxidant activity by potent scavenging actions on DPPH, ABTS, and nitric oxide free radicals. Overall results demonstrated that EPS of KMNTT-10 could be used in various industrial applications due to its excellent flocculation, emulsification potential, and good antioxidant activity.
ISSN:1566-2543
1572-8919
DOI:10.1007/s10924-020-01986-3