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Hydroxysafflor Yellow A Inhibits Staphylococcus aureus-Induced Mouse Endometrial Inflammation via TLR2-Mediated NF-kB and MAPK Pathway
The present study is designed to investigate the effect of hydroxysafflor yellow A (HYA) on Staphylococcus aureus ( S. aureus )-induced mouse endometrial inflammation and to explore its molecular mechanism. We established a mouse endometritis model by intrauterine injection of S. aureus and intraute...
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Published in: | Inflammation 2021-06, Vol.44 (3), p.835-845 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The present study is designed to investigate the effect of hydroxysafflor yellow A (HYA) on
Staphylococcus aureus
(
S. aureus
)-induced mouse endometrial inflammation and to explore its molecular mechanism. We established a mouse endometritis model by intrauterine injection of
S. aureus
and intrauterine injection of HYA for treatment. Immunohistochemistry, immunofluorescence, and Western blot were used to detect protein expression in uterine tissue, and qPCR was used to measure mRNA expression. HYA could significantly weak uterine pathological changes caused by
S. aureus
and reduce MPO activity, CD45, CD3, and ED-1 protein expression in uterine tissues of
S. aureus
-infected mice. Similarly, HYA also significantly decreased
S. aureus
induced the increase in TNF-α, IL-1β, and IL-6 in uterine tissue.
In vivo
, we found that knockdown of TLR2 was very important could significantly reduce
S. aureus
induced the elevated expression of TNF-α, IL-1β, and IL-6 in mEECs. Importantly, in terine tissues of
S. aureus
-infected mice, HYA significantly decreased the ratio of p-p65/p65, p-IKBα/IKBα, p-p38/p38, p-Erk/Erk, and p-JNK/JNK expression. HYA displays anti-inflammatory effects on
S. aureus
mouse endometrial inflammation, and this effect might be related to HYA which could block TLR2-mediated NF-kB and MAPK pathway. |
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ISSN: | 0360-3997 1573-2576 |
DOI: | 10.1007/s10753-020-01297-8 |