Pyriproxyfen Ingested With Attractive Toxic Sugar Baits Is Carried by the Feces and Reduces the Reproductive Potential of Adult Female Aedes aegypti (Diptera: Culicidae)

• Published in: Journal of medical entomology 2021-05, Vol.58 (3), p.1264-1269
• Main Authors: Pereira Silva, Tarsis Tamar, Teixeira, Aurea Vieira, Almeida e Silva, Alexandre de
• Format: Article
• Language: English
• Subjects: Aedes aegypti; Aquatic insects; Baits; Breeding sites; chemical control; Chemical properties; Control; Culicidae; Diptera; Excretion; fecal deposits; Feces; Fecundity; Females; Fertility; Filter paper; Ingestion; insect growth regulator (IGR); Insecticides; Intervals; Larvae; Mosquitoes; Physiological aspects; Pyriproxyfen; Reproductive sterilization; Residual effects; Sugar; VECTOR CONTROL, PEST MANAGEMENT, RESISTANCE, REPELLENTS
• ISSN: 0022-2585; 1938-2928
• DOI: 10.1093/jme/tjaa265

In the search for new strategies to control Aedes aegypti Linnaeus (Diptera: Culicidae), several studies have successfully related pyriproxyfen (PPF) tarsal transference to breeding sites (autodissemination), as well as the sterilization potential of females exposed to PPF. Potential PPF autodissemination by mosquito feces after the ingestion of sugar baits has also been proposed. Therefore, the present work evaluated several parameters, e.g., fecal production, residuality under dry and aqueous conditions, PPF excretion affecting emergence inhibition (EI) by fecal deposits of Ae. aegypti fed with attractive toxic sugar baits (ATSBs) containing PPF as well as their reproductive potential. Females were fed with ATSBs offered as droplets and the feces were collected using filter paper and transferred to plastic cups with L3 larvae to evaluate EI. The residual effect of feces in aqueous and dry conditions and PPF excretion on EI was obtained by keeping the feces in water or dried for different time intervals and using feces collected at 24-h intervals, respectively. Females received a bloodmeal after feeding on ATSBs, eggs and larval counting expressed the reproductive potential. The fecal mass was not affected by PPF concentration, but EI increased from 33 to 54% as the PPF concentration increased. The PPF excretion in the feces exceeded 96 h. The residual effect in the EI for feces kept in water was reduced by more than 60% after 30 d but was not affected under dry conditions. The fecundity and fertility of the females were reduced up to 51% and 97%, respectively.