Developing a nano-biosensor for early detection of pancreatic cancer

Purpose The purpose of this paper is to report on the development of a simple electrochemical nanobiosensor for early detection of pancreatic cancer. The nanobiosensor uses the newly emerged stable micro ribonucleic acid (miR-21) as a cancer-associated biomarker for diagnosis, prognosis or therapy r...

Full description

Saved in:
Bibliographic Details
Published in:Sensor review 2021-02, Vol.41 (1), p.93-100
Main Authors: Rawashdeh1, Isra, Al-Fandi, Mohamed Ghazi, Makableh, Yahia, Harahsha, Tasneem
Format: Article
Language:English
Subjects:
Atomic force microscopy
Biomarkers
Biopsy
Biosensors
Cancer
Electrons
Fourier transforms
Gold
Hybridization
Medical diagnosis
MicroRNAs
Multi wall carbon nanotubes
Nanoparticles
Pancreatic cancer
Potassium
Potentiostats
Ribonucleic acid
RNA
Spectrum analysis
Voltammetry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose The purpose of this paper is to report on the development of a simple electrochemical nanobiosensor for early detection of pancreatic cancer. The nanobiosensor uses the newly emerged stable micro ribonucleic acid (miR-21) as a cancer-associated biomarker for diagnosis, prognosis or therapy response. Design/methodology/approach The biosensing practice consists of two main steps: capturing probe immobilization on a working electrode modified with multi-walled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs) and then sensing the miR-21 interaction electrochemically. Two electrochemical techniques, atomic force microscopy and Fourier-transform infrared spectroscopy, were applied for characterizations. Findings The nanobiosensor sensitivity exhibited satisfying results to miR-21 and demonstrated a wide dynamic range with a detection limit of just about 3.68 femtomolar using the source measure unit (SMU). Originality/value Researchers commonly use potentiostats to perform the differential pulse voltammetry (DPV) measurements for the electrochemical biosensing applications. In this study, the SMU was used to perform the DPV to detect the biomarker miR-21 using the MWCNTs-AuNPs screen-printed electrode as the electrochemical system.
ISSN:0260-2288
1758-6828
DOI:10.1108/SR-01-2020-0004