Strain improvement of a new keratinolytic fungi

The SPFI fungus which was isolated from a poultry farm around Manipal, Udupi district was found to have keratinolytic activity which was observed using equivalent amount of tyrosine produced when the organism was introduced to the basal salt media containing 1% feather waste. After incubation, the a...

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2021, Vol.14 (2), p.843-845
Main Authors: Kamath, Venkatesh B, Hashim, Hawa, Keshri, Kamesh Kumar, Tripathy, Swati, Subrahmanyam, V. M.
Format: Article
Language:English
Subjects:
Enzymes
Fungi
Keratin
Morphology
Mutation
Poultry
Psoriasis
Reagents
Skin cancer
Ultraviolet radiation
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Summary:The SPFI fungus which was isolated from a poultry farm around Manipal, Udupi district was found to have keratinolytic activity which was observed using equivalent amount of tyrosine produced when the organism was introduced to the basal salt media containing 1% feather waste. After incubation, the absorbance was measured using the colorimeter at 660nm and the graph of absorbance against concentration was plotted. iii.Keratinolytic activity of the fungal (SPF1) isolates: 2.5ml of the fungal suspension in distilled water was made from the previously preserved SDA slants and inoculated in 75ml of the Basal salt media which contained 1% feather waste. The SPF1 strain from the previously preserved slants was taken and a 15ml suspension in sterile water was made and kept in a petri dish of 80mm in diameter and exposed to UV rays for 6,8 and 10 minutes at a distance of 25cm from the UV lamp.
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.5958/0974-360X.2021.00149.9