Ecotoxicological risk assessment of pentachlorophenol, an emerging DBP to plants: evaluation of oxidative stress and antioxidant responses

• Published in: Environmental science and pollution research international 2021-06, Vol.28 (22), p.27954-27965
• Main Authors: Ranjan, Jyoti, Joshi, Vayam, Mandal, Tamale, Mandal, Dalia Dasgupta
• Format: Article
• Language: English
• Subjects: Acids; Antioxidants; Aquatic Pollution; Ascorbic acid; Atmospheric Protection/Air Quality Control/Air Pollution; Carboxylic acids; Catalase; Chlorophenol; Contaminants; Crop production; Defense mechanisms; Disinfection; Disinfection & disinfectants; DNA fragmentation; Drinking water; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Evaluation; Genotoxicity; Glutathione; Glutathione peroxidase; Hydrogen peroxide; L-Ascorbate peroxidase; Lignin; Lipid peroxidation; Lipids; Oxidative stress; Pentachlorophenol; Peroxidase; Peroxidation; Plant species; Research Article; Risk assessment; Toxicity; Waste Water Technology; Water Management; Water Pollution Control; Water treatment; Water treatment plants
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-021-12578-6

Chlorophenols are not only noticed in an effluvium of industries but also can emerge from the water treatment plants for domestic supply which poses a high threat for crop production and human health. Therefore, research on their risks to ecosystem and human health via ecotoxicological tests to derivate permissible environmental contaminant concentrations is necessary. The chlorophenols produced in the course of chlorination of potable water is an outcome of natural carboxylic acids/organic material and those chlorophenols occurred as emerging disinfection byproducts (EDBPs). Among chlorophenols, pentachlorophenol (PCP) has been recently identified as one of the important EDBPs. The main objective was to evaluate the PCP-induced genotoxicity and the oxidative damage in two plant species, i.e., Allium cepa and Vigna radiata. Genotoxicity of PCP was examined at three selected concentrations based on EC 50 (half-maximal effective concentrations) values in both the plants along with the defense mechanism. EC 50 value for A. cepa and V. radiata was 0.7 mg/L and 35 mg/L. Root length inhibition, DNA laddering, lipid peroxidation, H 2 O 2 content, and antioxidant enzymatic assays evaluated revealed a dose-dependent response. PCP influenced defense enzyme glutathione peroxidase (GPX) and ascorbate peroxidase (APX) action in both plants and showed deprivement of catalase (CAT) with the increase of PCP concentrations. PCP-invaded toxicity management by these plants implied that A. cepa is more sensitive than V. radiata regarding PCP-induced toxicity.