Characterization, antioxidant and antibacterial activity of cultivated sea cucumbers from Bali, Indonesia

• Published in: IOP conference series. Earth and environmental science 2021-04, Vol.744 (1), p.12102
• Main Authors: Wulandari, D A, Murniasih, T, Sari, M, Syahputra, G, Rasyid, A, Septiana, E, Untari, F, Harahap, I A, Ardiansyah, A, Gustini, N, Putra, M Y
• Format: Article
• Language: English
• Subjects: Antibacterial activity; Antioxidants; Bacteria; Cadmium; Carbohydrates; E coli; Ethanol; Heavy metal content; Heavy metals; Holothuroidea; Invertebrates; Lead; Mercury (metal); Vegetables
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/744/1/012102

Sea cucumber is marine commodity widely distributed throughout Indonesian waters, Indonesia exported sea cucumber to Hongkong, Japan, Korea, Singapore, Taiwan, Malaysia and Australia. This study aims to characterize sea cucumber, determine the antioxidant, and antibacterial activity of cultivated sea cucumbers. Sea cucumbers are cultivated in the pond for 12 months, with the weight and size average 160.9 grams, and 15.3 cm at harvest. The sea cucumbers were characterized by measuring their proximate and heavy metals content. The samples were extracted using water at 60°C, 70°C, 80°C, and soxhletation with 70% ethanol. Furthermore, the antibacterial analysis was tested on three bacteria, namely Staphylococcus aureus, Bacillus subtilis, and Escherichia coli and antioxidants were analyzed using the ABTS method. The results showed that the sea cucumber protein content was 78.77%, fat content was 1.66%, and carbohydrate was 3.80%. Heavy metal content of cultivated sea cucumbers, including as 1.12 mg/kg, Pb 0.15 mg/kg, Ni, Hg, and Cd were not detected. Antibacterial test results showed all the extract of sea cucumber could not inhibit the growth of bacteria. The best antioxidant activity was found in dried sea cucumbers extracted using the soxhletation method with IC50 value 62.72 μg/mL.