Potassium-regulated Immobilization of Cortisol Aptamer for Field-effect Transistor Biosensor to Detect Changes in Charge Distribution with Aptamer Transformation

Salivary cortisol concentration and its circadian variation were detected by optimizing the ionic concentration of a solution during the immobilization of aptamers on a field-effect transistor biosensor. This was achieved by modifying our previously developed technique of detecting uncharged cortiso...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry letters 2021-05, Vol.50 (5), p.892-895
Main Authors: Kuroiwa, Shigeki, Hayashi, Hiroki, Toyama, Ryo, Kaneko, Naoto, Horii, Katsunori, Ohashi, Keishi, Momma, Toshiyuki, Osaka, Tetsuya
Format: Article
Language:English
Subjects:
Biosensors
Charge distribution
Field effect transistors
Hormones
Immobilization
Semiconductor devices
Transistors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Salivary cortisol concentration and its circadian variation were detected by optimizing the ionic concentration of a solution during the immobilization of aptamers on a field-effect transistor biosensor. This was achieved by modifying our previously developed technique of detecting uncharged cortisol using the transformation of negatively charged aptamers by controlling the aptamer spacing. This spacing control was achieved by synthesizing pseudo-cortisol-binding aptamers with a guanine-quadruplex during the immobilization of the aptamers in a highly concentrated solution of K+.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.200876