Complete chloroplast genome of novel Adinandra megaphylla Hu species: molecular structure, comparative and phylogenetic analysis

Adinandra megaphylla Hu is a medicinal plant belonging to the Adinandra genus, which is well-known for its potential health benefits due to its bioactive compounds. This study aimed to assemble and annotate the chloroplast genome of A. megaphylla as well as compare it with previously published cp ge...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2021-06, Vol.11 (1)
Main Authors: Nguyen, Huu Quan, Nguyen, Thi Ngoc Lan, Doan, Thi Nhung, Nguyen, Thi Thu Nga, Phạm, Mai Huong, Le, Tung Lam, Sy, Danh Thuong, Chu, Hoang Ha, Chu, Hoang Mau
Format: Article
Language:English
Subjects:
631/337
631/449
631/61
Bioactive compounds
Chloroplasts
DNA sequencing
Genetic relationship
Genomes
Humanities and Social Sciences
Medicinal plants
multidisciplinary
Phylogenetics
Phylogeny
rRNA
Science
Science (multidisciplinary)
Transfer RNA
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adinandra megaphylla Hu is a medicinal plant belonging to the Adinandra genus, which is well-known for its potential health benefits due to its bioactive compounds. This study aimed to assemble and annotate the chloroplast genome of A. megaphylla as well as compare it with previously published cp genomes within the Adinandra genus. The chloroplast genome was reconstructed using de novo and reference-based assembly of paired-end reads generated by long-read sequencing of total genomic DNA. The size of the chloroplast genome was 156,298 bp, comprised a large single-copy (LSC) region of 85,688 bp, a small single-copy (SSC) region of 18,424 bp, and a pair of inverted repeats (IRa and IRb) of 26,093 bp each; and a total of 51 SSRs and 48 repeat structures were detected. The chloroplast genome includes a total of 131 functional genes, containing 86 protein-coding genes, 37 transfer RNA genes, and 8 ribosomal RNA genes. The A. megaphylla chloroplast genome indicated that gene content and structure are highly conserved. The phylogenetic reconstruction using complete cp sequences, matK and trnL genes from Pentaphylacaceae species exhibited a genetic relationship. Among them, matK sequence is a better candidate for phylogenetic resolution. This study is the first report for the chloroplast genome of the A. megaphylla .
ISSN:2045-2322
DOI:10.1038/s41598-021-91071-z