Immunochromatographic Test Systems for Detection of Microcystin-LR in Seafood

For the rapid control of the highly toxic phycotoxin microcystin-LR in seafood, immunochromatographic test systems have been developed and compared using two types of gold nanoparticles as markers, namely, spherical particles and nanoflowers. The test systems are based on an indirect competitive ass...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2021-05, Vol.57 (3), p.403-409
Main Authors: Zvereva, E. A., Hendrickson, O. D., Zherdev, A. V., Dzantiev, B. B.
Format: Article
Language:English
Subjects:
Antibodies
Antigen-antibody complexes
Biochemistry
Biomedical and Life Sciences
Contamination
Detection limits
Gold
Life Sciences
Medical Microbiology
Microbiology
Microcystin-LR
Microcystins
Mussels
Nanoparticles
Pollution control
Sample preparation
Seafood
Test systems
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Summary:For the rapid control of the highly toxic phycotoxin microcystin-LR in seafood, immunochromatographic test systems have been developed and compared using two types of gold nanoparticles as markers, namely, spherical particles and nanoflowers. The test systems are based on an indirect competitive assay, in which the detectable immune complexes include native microcystin-LR-specific antibodies and nanoparticle-labeled anti-species antibodies. The developed test systems allow for the detection of microcystin-LR within 18 min with instrumental detection limits of 0.2 and 0.1 ng/mL when using spherical nanoparticles and gold nanoflowers, respectively. The visual detection limit is 1 ng/mL for both formats. The parameters of nanoparticles, which determine the analytical characteristics of the corresponding test systems are characterized. The applicability of the developed test systems for the control of contamination of fish and seafood (shrimp, octopus, squid, and mussels) combining rapid stages of sample preparation and immunochromatography has been shown.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683821030170