Affirmation of the Separate Rate-law Method for Determining vmax and KM of the Michaelis Reaction

• Published in: Journal of physics. Conference series 2021-05, Vol.1912 (1), p.12005
• Main Authors: Patiha, Saraswati, T E, Wahyuningsih, S, Firdaus, M, Masykur, A
• Format: Article
• Language: English
• Subjects: Methods; Physics
• ISSN: 1742-6588; 1742-6596
• DOI: 10.1088/1742-6596/1912/1/012005

The maximum-velocity, v max , and the Michaelis-constant, K M , are the essence of the Michaelis-Menten equation. The value of v max is determined when the reaction is in zeroth -order and K M is in first-order . With a slight change in the notion of v max concept, both were used as the basis for a new determination method called Separate Rate-law Method ( SRlM ). As yet, the Lineweaver-Burk and Eadie-methods were the most commonly used. Both combine zeroth -order and first -order data. The purpose of this study was to validate the quality of all methods. The research was carried out theoretically. All were tested by applying them to the same literature data, and the results were compared. The assessment is based on the v max and K M values obtained; doubling [ E ] will double v max but the same K M . The results show that the two present methods are inaccurate. Both give v max and K M values even if the reaction is always first -order. Based on the integral method, two variants of the new method, SRlM IM , and initial rate, SRlM IRM , were also introduced. All new methods give more accuracy, which results in the method accuracy of SRlM ≈ SRlM IM > SRlM IRM .