Use of Total Fluorescence Spectroscopy for the Highly Sensitive Simultaneous Determination of Fluoroquinolones in Rabbit Plasma

A method for the quantification of a mixture of fluorescent antibiotics with broad, highly overlapped excitation and emission spectra is proposed. The method enables the simultaneous determination of sarafloxacin, flumequine and ofloxacin by using the fluorescence excitation-emission matrix and perf...

Full description

Saved in:
Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2021-06, Vol.76 (6), p.733-741
Main Authors: Pulgarín, José Antonio Murillo, Molina, Aurelia Alañón, Boras, Narjisse
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antibiotics
Chemistry
Chemistry and Materials Science
Emission spectra
Excitation
Excitation spectra
Fluorescence
Fluorescence spectroscopy
Fluorimetry
Mathematical analysis
Ofloxacin
Selectivity
Spectrum analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method for the quantification of a mixture of fluorescent antibiotics with broad, highly overlapped excitation and emission spectra is proposed. The method enables the simultaneous determination of sarafloxacin, flumequine and ofloxacin by using the fluorescence excitation-emission matrix and performing two different scans, namely, a linear variable angle synchronous fluorimetry scan at 15.6° and an emission scan at λ exc of 320 nm. This ensures complete resolution of the mixture without the need for a separation technique. Various factors influencing the fluorescence intensity were assessed in order to maximize the sensitivity and obtain an adequate selectivity. The method was successfully used to determine the analytes in pharmaceuticals and rabbit plasma. The results obtained compared favorably with those provided by HPLC.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934821060101