Pyrazole Derivative Attenuates Store-Dependent Ca2+ Entry in Rat Peritoneal Macrophages

— Store-dependent Ca 2+ entry is a ubiquitous mechanism of regulated Ca 2+ entry into eukaryotic cells. It is activated upon depletion of intracellular Ca 2+ stores and is involved in the regulation of a wide range of cellular processes. To elucidate the pharmacological characteristics of store-depe...

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Bibliographic Details
Published in:Cell and tissue biology 2021, Vol.15 (3), p.293-300
Main Authors: Milenina, L. S., Krutetskaya, Z. I., Antonov, V. G., Krutetskaya, N. I.
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Biomedical and Life Sciences
Ca2+-transporting ATPase
Calcium (intracellular)
Calcium influx
Cell Biology
Cyclopiazonic acid
Immunomodulation
Life Sciences
Macrophages
Peritoneum
Thapsigargin
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Summary:— Store-dependent Ca 2+ entry is a ubiquitous mechanism of regulated Ca 2+ entry into eukaryotic cells. It is activated upon depletion of intracellular Ca 2+ stores and is involved in the regulation of a wide range of cellular processes. To elucidate the pharmacological characteristics of store-dependent Ca 2+ entry into cells, we studied the effect of YM-58483, pyrazole derivative immunosuppressant, on the store-dependent Ca 2+ entry in rat peritoneal macrophages induced by the endoplasmic Ca 2+ -ATPase inhibitors thapsigargin and cyclopiazonic acid, as well as the disulfide-containing immunomodulators glutoxim and molixan. Using Fura-2AM, fluorescent calcium indicator, it has been shown for the first time that, in rat peritoneal macrophages, as well as in other cell types, pyrazole derivative YM-58483 effectively inhibits store-dependent Ca 2+ entry and is a useful pharmacological tool to study the store-dependent Ca 2+ entry in macrophages. The data obtained additionally confirm that Ca 2+ entry induced by glutoxim or molixan is realized via the store-dependent mechanism.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X21030068