EFFECT OF THE VEGETATION CYCLE ON TOTAL PHENOLIC AND FLAVONOID COMPOUNDS IN Hypericum perforatum L. AND Melissa officinalis L. COLLECTED IN MONTENEGRO

• Published in: Poljoprivreda i šumarstvo 2021, Vol.67 (1), p.181-190
• Main Authors: SLADjANA, KRIVOKAPIC, TIJANA, PEJATOVIC
• Format: Article
• Language: English
• Subjects: Acids; Antibacterial activity; Bioactive compounds; Cytotoxicity; Epidermis; Essential oils; Evaporators; Flavonoids; Glycosides; Investigations; Kaempferol; Phenolic compounds; Phenols; Polyphenols; Quercetin; Vegetation; Vegetation effects; Water baths
• ISSN: 0554-5579; 1800-9492
• DOI: 10.17707/AgricultForest.67.1.15

Keywords: total phenolic content, total flavonoids content, Hypericum L., Melissa L. INTRODUCTION Plants are producing a multitude of bioactive compounds (Dixon, 2001), among them polyphenols like phenolics and flavonoids, that have been a limitless source of experimental analysis (Sujana et al., 2013) and which also have numerous different functions (Halliwell 2006, Albayrak et al., 2010, Sarbu et al., 2019, Obložinský et al., 2006). H. perforatum L. is well recognized when it comes to flavonoids content (quercetin and kaempferol at the first place) (Nahrstedt and Butterweck, 2010) which are found in its leaves as glycosides located in special compartments of the cells of epidermis, and which are responsible for UV-protection (Germ, et al., 2010). In the literature there are several studies about phytochemical content, antimicrobial, antimutagenic and cytotoxic activity of essential oils from plants growing in Montenegro (Šćepanović et al., 2019, Damjanović-Vratnica et al., 2015, Bošković, et al., 2018, Božović et al., 2015, 2018; Artini et la., 2018, Tadić et al., 2017, Stešević et al., 2016, Vuković-Gačić et al., 2006), while the effect of vegetation cycle on chemical content and antibacterial activity of essential oil was investigated by Damjanović-Vratnica et al., (2011) for the Satureja montana L. species. The plant material was determined in the laboratory of the Study program for Biology, Faculty of Natural Sciences and Mathematics, University of Montenegro, and genus and species of the plant were identified and confirmed. a)Aparatus For the purpose of extraction water bath from the manufacturer Vims elektrik was used, and after the extraction, sand bath and automated rotor-vacuum evaporator IKA rv 10 were used for removing dissolvent from the extract.