Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. k...

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Bibliographic Details
Published in:Bulletin of the Veterinary Institute in Puławy 2014-01, Vol.58 (2), p.219-222
Main Authors: Nadăş, George Cosmin, Kalmár, Zsuzsa, Taulescu, Marian Aurel, Chirilă, Flore, Bouari, Cosmina Maria, Răpuntean, Sorin, Bolfa, Pompei, Fiţ, Nicodim Iosif
Format: Article
Language:English
Subjects:
Candida
Candida krusei
Chromatic Candida
Genotyping
identification
PCR-RFLP
Polymerase chain reaction
Restriction fragment length polymorphism
Romania
Strains (organisms)
test API 20 C AUX system
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Summary:The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.
ISSN:2300-3235
2450-7393
2300-3235
2450-8608
DOI:10.2478/bvip-2014-0032