Optimisation of protocol for Clostridium botulinum detection in mink feed

As the test material mink feed with natural microflora was used. The analyses were conducted using Wrzosek and TPGY broth media, and Willis–Hobbs and Zeissler differential agar media. Wrzosek, Willis–Hobbs, and Zeissler media are described in Polish Standards approved by the National Standards Body...

Full description

Saved in:
Bibliographic Details
Published in:Bulletin of the Veterinary Institute in Puławy 2015-09, Vol.59 (3), p.377-382
Main Authors: Grenda, Tomasz, Kukier, Elżbieta, Goldsztejn, Magdalena, Kwiatek, Krzysztof, Kozieł, Nina
Format: Article
Language:English
Subjects:
Agar
Anaerobic bacteria
culture techniques
Differential media
feed
Lethality
Microflora
mink
real-time PCR
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:As the test material mink feed with natural microflora was used. The analyses were conducted using Wrzosek and TPGY broth media, and Willis–Hobbs and Zeissler differential agar media. Wrzosek, Willis–Hobbs, and Zeissler media are described in Polish Standards approved by the National Standards Body in Poland and routinely used in detection of anaerobic bacteria in Poland. Detection and identification of was performed with a previously validated real-time PCR method based on gene detection, which is common in all toxotypes. The use of Wrzosek broth and Zeissler agar in routine analyses for detection and identification of was ineffective and limited. The obtained results showed the highest culturing process effectiveness in TPGY broth with 72 h incubation at 30°C and isolation on Willis–Hobbs agar. The real-time PCR method based on gene detection used in this study could be utilised as a supplementary tool to the mouse lethality assay.
ISSN:2300-3235
2450-7393
2300-3235
2450-8608
DOI:10.1515/bvip-2015-0056