Liquid chromatography – tandem mass spectrometry method for the determination of ten tetracycline residues in muscle samples

A liquid chromatography – tandem mass spectrometry (LC-MS/MS) method for the determination of oxytetracycline (OTC), 4-epi oxytetracycline (4-epi OTC), tetracycline (TC), 4-epi tetracycline (4-epi TC), chlortetracycline (CTC), 4-epi chlortetracycline (4-epi CTC), doxycycline (DC), minocycline (MINO)...

Full description

Saved in:
Bibliographic Details
Published in:Bulletin of the Veterinary Institute in Puławy 2015-09, Vol.59 (3), p.345-352
Main Authors: Gajda, Anna, Posyniak, Andrzej
Format: Article
Language:English
Subjects:
Acetonitrile
Acids
Antibiotics
Chlortetracycline
Chromatography
Doxycycline
Formic acid
Ions
LC-MS/MS
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Methacycline
method validation
Minocycline
Muscles
Oxalic acid
Oxytetracycline
Rolitetracycline
Scientific imaging
Tetracyclines
Trichloroacetic acid
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c360t-f91608d6e9cbdcd7ceee9aacbdae6fe1deb86d0274c1fce327f496809f3919923
cites
container_end_page 352
container_issue 3
container_start_page 345
container_title Bulletin of the Veterinary Institute in Puławy
container_volume 59
creator Gajda, Anna
Posyniak, Andrzej
description A liquid chromatography – tandem mass spectrometry (LC-MS/MS) method for the determination of oxytetracycline (OTC), 4-epi oxytetracycline (4-epi OTC), tetracycline (TC), 4-epi tetracycline (4-epi TC), chlortetracycline (CTC), 4-epi chlortetracycline (4-epi CTC), doxycycline (DC), minocycline (MINO), methacycline (META) and rolitetracycline (ROLI) residues in muscles was developed. The procedure consisted of an oxalic acid extraction followed by protein removal with trichloroacetic acid. Further solid phase clean-up on polymeric (Strata X) reversed phase columns was performed to obtain an extract suitable for LC-MS/MS analysis. The tetracyclines were separated on a C 18 analytical column with mobile phase consisting of 0.01% formic acid in acetonitrile and 0.01% formic acid in water in gradient mode. The method was validated according to the Commission Decision 2002/657/EC. The recoveries of all target compounds were 91.8% – 103.6%. The decision limits were from 109.0 to 119.8 μg/kg and detection capability varied within the range of 122.2 to 137.6 μg/kg, depending on the analyte.
doi_str_mv 10.1515/bvip-2015-0051
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2545235286</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2545235286</sourcerecordid><originalsourceid>FETCH-LOGICAL-c360t-f91608d6e9cbdcd7ceee9aacbdae6fe1deb86d0274c1fce327f496809f3919923</originalsourceid><addsrcrecordid>eNptkMtKxDAUhosoOI5uXQdcV5Om6TTgRgZvMOBG1yWTnEwztE0nSZVuxHfwDX0SU0bQhYtzg_8_5_AlyTnBl4QRdrV-NX2aYcJSjBk5SGYZxTilGWWHf_rj5MT7LcZ5ifN8lryvzG4wCsna2VYEu3Gir0f09fGJgugUtKgV3iPfgwxRAcGNKObaKqStQ6EGpCCAa00ngrEdshoF6GIEJ-QoG9MBcuCNGsAj06F28LIB5EXbN-BPkyMtGg9nP3WevNzdPi8f0tXT_ePyZpVKWuCQak4KXKoCuFwrqRYSALgQcRBQaCAK1mWhcLbIJdESaLbQOS9KzDXlhPOMzpOL_d7e2V38JFRbO7gunqwylrPIJSuLqLrcq6Sz3jvQVe9MK9xYEVxNjKuJcTUxribG0XC9N7yJJkJQsHHDGJvf7f8bGac0Z_QbN3WIxQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2545235286</pqid></control><display><type>article</type><title>Liquid chromatography – tandem mass spectrometry method for the determination of ten tetracycline residues in muscle samples</title><source>Publicly Available Content Database</source><creator>Gajda, Anna ; Posyniak, Andrzej</creator><creatorcontrib>Gajda, Anna ; Posyniak, Andrzej</creatorcontrib><description>A liquid chromatography – tandem mass spectrometry (LC-MS/MS) method for the determination of oxytetracycline (OTC), 4-epi oxytetracycline (4-epi OTC), tetracycline (TC), 4-epi tetracycline (4-epi TC), chlortetracycline (CTC), 4-epi chlortetracycline (4-epi CTC), doxycycline (DC), minocycline (MINO), methacycline (META) and rolitetracycline (ROLI) residues in muscles was developed. The procedure consisted of an oxalic acid extraction followed by protein removal with trichloroacetic acid. Further solid phase clean-up on polymeric (Strata X) reversed phase columns was performed to obtain an extract suitable for LC-MS/MS analysis. The tetracyclines were separated on a C 18 analytical column with mobile phase consisting of 0.01% formic acid in acetonitrile and 0.01% formic acid in water in gradient mode. The method was validated according to the Commission Decision 2002/657/EC. The recoveries of all target compounds were 91.8% – 103.6%. The decision limits were from 109.0 to 119.8 μg/kg and detection capability varied within the range of 122.2 to 137.6 μg/kg, depending on the analyte.</description><identifier>ISSN: 2300-3235</identifier><identifier>ISSN: 2450-7393</identifier><identifier>EISSN: 2300-3235</identifier><identifier>EISSN: 2450-8608</identifier><identifier>DOI: 10.1515/bvip-2015-0051</identifier><language>eng</language><publisher>Pulawy: Sciendo</publisher><subject>Acetonitrile ; Acids ; Antibiotics ; Chlortetracycline ; Chromatography ; Doxycycline ; Formic acid ; Ions ; LC-MS/MS ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Methacycline ; method validation ; Minocycline ; Muscles ; Oxalic acid ; Oxytetracycline ; Rolitetracycline ; Scientific imaging ; Tetracyclines ; Trichloroacetic acid</subject><ispartof>Bulletin of the Veterinary Institute in Puławy, 2015-09, Vol.59 (3), p.345-352</ispartof><rights>2015. This work is published under http://creativecommons.org/licenses/by-nc-nd/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-f91608d6e9cbdcd7ceee9aacbdae6fe1deb86d0274c1fce327f496809f3919923</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2545235286?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,44590</link.rule.ids></links><search><creatorcontrib>Gajda, Anna</creatorcontrib><creatorcontrib>Posyniak, Andrzej</creatorcontrib><title>Liquid chromatography – tandem mass spectrometry method for the determination of ten tetracycline residues in muscle samples</title><title>Bulletin of the Veterinary Institute in Puławy</title><description>A liquid chromatography – tandem mass spectrometry (LC-MS/MS) method for the determination of oxytetracycline (OTC), 4-epi oxytetracycline (4-epi OTC), tetracycline (TC), 4-epi tetracycline (4-epi TC), chlortetracycline (CTC), 4-epi chlortetracycline (4-epi CTC), doxycycline (DC), minocycline (MINO), methacycline (META) and rolitetracycline (ROLI) residues in muscles was developed. The procedure consisted of an oxalic acid extraction followed by protein removal with trichloroacetic acid. Further solid phase clean-up on polymeric (Strata X) reversed phase columns was performed to obtain an extract suitable for LC-MS/MS analysis. The tetracyclines were separated on a C 18 analytical column with mobile phase consisting of 0.01% formic acid in acetonitrile and 0.01% formic acid in water in gradient mode. The method was validated according to the Commission Decision 2002/657/EC. The recoveries of all target compounds were 91.8% – 103.6%. The decision limits were from 109.0 to 119.8 μg/kg and detection capability varied within the range of 122.2 to 137.6 μg/kg, depending on the analyte.</description><subject>Acetonitrile</subject><subject>Acids</subject><subject>Antibiotics</subject><subject>Chlortetracycline</subject><subject>Chromatography</subject><subject>Doxycycline</subject><subject>Formic acid</subject><subject>Ions</subject><subject>LC-MS/MS</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Methacycline</subject><subject>method validation</subject><subject>Minocycline</subject><subject>Muscles</subject><subject>Oxalic acid</subject><subject>Oxytetracycline</subject><subject>Rolitetracycline</subject><subject>Scientific imaging</subject><subject>Tetracyclines</subject><subject>Trichloroacetic acid</subject><issn>2300-3235</issn><issn>2450-7393</issn><issn>2300-3235</issn><issn>2450-8608</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNptkMtKxDAUhosoOI5uXQdcV5Om6TTgRgZvMOBG1yWTnEwztE0nSZVuxHfwDX0SU0bQhYtzg_8_5_AlyTnBl4QRdrV-NX2aYcJSjBk5SGYZxTilGWWHf_rj5MT7LcZ5ifN8lryvzG4wCsna2VYEu3Gir0f09fGJgugUtKgV3iPfgwxRAcGNKObaKqStQ6EGpCCAa00ngrEdshoF6GIEJ-QoG9MBcuCNGsAj06F28LIB5EXbN-BPkyMtGg9nP3WevNzdPi8f0tXT_ePyZpVKWuCQak4KXKoCuFwrqRYSALgQcRBQaCAK1mWhcLbIJdESaLbQOS9KzDXlhPOMzpOL_d7e2V38JFRbO7gunqwylrPIJSuLqLrcq6Sz3jvQVe9MK9xYEVxNjKuJcTUxribG0XC9N7yJJkJQsHHDGJvf7f8bGac0Z_QbN3WIxQ</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Gajda, Anna</creator><creator>Posyniak, Andrzej</creator><general>Sciendo</general><general>De Gruyter Poland</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20150901</creationdate><title>Liquid chromatography – tandem mass spectrometry method for the determination of ten tetracycline residues in muscle samples</title><author>Gajda, Anna ; Posyniak, Andrzej</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-f91608d6e9cbdcd7ceee9aacbdae6fe1deb86d0274c1fce327f496809f3919923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acetonitrile</topic><topic>Acids</topic><topic>Antibiotics</topic><topic>Chlortetracycline</topic><topic>Chromatography</topic><topic>Doxycycline</topic><topic>Formic acid</topic><topic>Ions</topic><topic>LC-MS/MS</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Methacycline</topic><topic>method validation</topic><topic>Minocycline</topic><topic>Muscles</topic><topic>Oxalic acid</topic><topic>Oxytetracycline</topic><topic>Rolitetracycline</topic><topic>Scientific imaging</topic><topic>Tetracyclines</topic><topic>Trichloroacetic acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gajda, Anna</creatorcontrib><creatorcontrib>Posyniak, Andrzej</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Health &amp; Medical Collection (ProQuest Medical &amp; Health Databases)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Bulletin of the Veterinary Institute in Puławy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gajda, Anna</au><au>Posyniak, Andrzej</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid chromatography – tandem mass spectrometry method for the determination of ten tetracycline residues in muscle samples</atitle><jtitle>Bulletin of the Veterinary Institute in Puławy</jtitle><date>2015-09-01</date><risdate>2015</risdate><volume>59</volume><issue>3</issue><spage>345</spage><epage>352</epage><pages>345-352</pages><issn>2300-3235</issn><issn>2450-7393</issn><eissn>2300-3235</eissn><eissn>2450-8608</eissn><abstract>A liquid chromatography – tandem mass spectrometry (LC-MS/MS) method for the determination of oxytetracycline (OTC), 4-epi oxytetracycline (4-epi OTC), tetracycline (TC), 4-epi tetracycline (4-epi TC), chlortetracycline (CTC), 4-epi chlortetracycline (4-epi CTC), doxycycline (DC), minocycline (MINO), methacycline (META) and rolitetracycline (ROLI) residues in muscles was developed. The procedure consisted of an oxalic acid extraction followed by protein removal with trichloroacetic acid. Further solid phase clean-up on polymeric (Strata X) reversed phase columns was performed to obtain an extract suitable for LC-MS/MS analysis. The tetracyclines were separated on a C 18 analytical column with mobile phase consisting of 0.01% formic acid in acetonitrile and 0.01% formic acid in water in gradient mode. The method was validated according to the Commission Decision 2002/657/EC. The recoveries of all target compounds were 91.8% – 103.6%. The decision limits were from 109.0 to 119.8 μg/kg and detection capability varied within the range of 122.2 to 137.6 μg/kg, depending on the analyte.</abstract><cop>Pulawy</cop><pub>Sciendo</pub><doi>10.1515/bvip-2015-0051</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2300-3235
ispartof Bulletin of the Veterinary Institute in Puławy, 2015-09, Vol.59 (3), p.345-352
issn 2300-3235
2450-7393
2300-3235
2450-8608
language eng
recordid cdi_proquest_journals_2545235286
source Publicly Available Content Database
subjects Acetonitrile
Acids
Antibiotics
Chlortetracycline
Chromatography
Doxycycline
Formic acid
Ions
LC-MS/MS
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Methacycline
method validation
Minocycline
Muscles
Oxalic acid
Oxytetracycline
Rolitetracycline
Scientific imaging
Tetracyclines
Trichloroacetic acid
title Liquid chromatography – tandem mass spectrometry method for the determination of ten tetracycline residues in muscle samples
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T13%3A54%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Liquid%20chromatography%20%E2%80%93%20tandem%20mass%20spectrometry%20method%20for%20the%20determination%20of%20ten%20tetracycline%20residues%20in%20muscle%20samples&rft.jtitle=Bulletin%20of%20the%20Veterinary%20Institute%20in%20Pu%C5%82awy&rft.au=Gajda,%20Anna&rft.date=2015-09-01&rft.volume=59&rft.issue=3&rft.spage=345&rft.epage=352&rft.pages=345-352&rft.issn=2300-3235&rft.eissn=2300-3235&rft_id=info:doi/10.1515/bvip-2015-0051&rft_dat=%3Cproquest_cross%3E2545235286%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c360t-f91608d6e9cbdcd7ceee9aacbdae6fe1deb86d0274c1fce327f496809f3919923%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2545235286&rft_id=info:pmid/&rfr_iscdi=true