Acute exposure to microcystin-LR induces hepatopancreas toxicity in the Chinese mitten crab (Eriocheir sinensis)

• Published in: Archives of toxicology 2021-07, Vol.95 (7), p.2551-2570
• Main Authors: Chi, Cheng, Yu, Xia-Wei, Zhang, Cai-Yan, Liu, Jia-Dai, Ye, Ming-Wen, Zhang, Ding-Dong, Liu, Wen-Bin
• Format: Article
• Language: English
• Subjects: Antioxidants; Apoptosis; Aquaculture; BAX protein; Bcl-2 protein; Biologics; Biomedical and Life Sciences; Biomedicine; Breeding; Caspase-3; Cyanobacteria; Environmental Health; Epidemics; Eriocheir sinensis; Exposure; Farm ponds; Flavor; Gene expression; Glutathione; Glutathione peroxidase; Glutathione reductase; Glutathione transferase; Hepatopancreas; Hepatotoxicity; Lipids; Microcystin-LR; Necrosis; Nutritive value; Occupational Medicine/Industrial Medicine; Outbreaks; Peroxidase; Pharmacology/Toxicology; Reactive oxygen species; Reductases; Structural damage; Superoxide dismutase; Toxicity
• ISSN: 0340-5761; 1432-0738
• DOI: 10.1007/s00204-021-03061-9

The Chinese mitten crab is an important economic species in the Chinese aquaculture industry due to its rich nutritional value and distinct flavor. The hepatopancreas is a popular edible part of the Chinese mitten crab, and therefore, hepatopancreatic health directly determines its quality. However, a large-scale outbreak of hepatopancreatic necrosis syndrome (“Shuibiezi” disease in Chinese), which is caused by abiotic agents correlated with cyanobacteria bloom outbreaks, adversely affects the Chinese mitten crab breeding industry. Cyanobacterial blooms that occur in high-density farming ponds can produce microcystin-LR (MC-LR), which is hepatotoxic in fish and mammals. Hepatopancreas toxicity of MC-LR (0, 25, 50 and 75 μg/kg) was investigated after 48 h of exposure. The MC-LR can cause hepatopancreatic injury by inducing hepatopancreatic structural damage, subcellular structural changes, and cell apoptosis, followed by enhanced lipid peroxidase, reactive oxygen species, and apoptosis-related enzyme (Caspase 3, 8, and 9) activities. These in turn promote gene and protein expression of apoptosis-associated proteases (Caspase 3, 7, and 8, Bcl-2, and Bax), and alter antioxidant system responses (superoxide dismutase, glutathione S-transferase, glutathione peroxidase, glutathione reductase activities, and glutathione content). The present study is the first report on MC-LR hepatotoxicity in the Chinese mitten crab and confirms hepatopancreas toxicity, providing a theoretical basis for enhancing MCs resistance and developing preventive and curative measures against hepatopancreatic disease in the Chinese mitten crab breeding industry.