Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation

• Published in: Phyton (Buenos Aires) 2021, Vol.90 (6), p.1741-1748
• Main Authors: Arias-P閞ez, Eliana, Alberto Lecona-Guzm醤, Carlos, Antonio Guti閞rez-Miceli, Federico, Adolfo Montes-Molina, Joaqu韓, Ruiz-Lau, Nancy
• Format: Article
• Language: English
• Subjects: 2,4-D; Alginic acid; Benzyladenine; Calcium chloride; Coffea arabica; Coffee; Embryonic growth stage; Embryos; Encapsulation; Explants; Fluorescein; Fluorescein diacetate; Genotypes; Indoleacetic acid; Sodium alginate; Somatic embryogenesis
• ISSN: 1851-5657; 0031-9457; 1851-5657
• DOI: 10.32604/phyton.2021.016004

The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos (Coffea arabica L.), for the conservation of genotypes with characteristics of commercial interest. Somatic embryos were induced from leaf explants in Murashige and Skoog medium (MS) supplemented with 1 mg · L−1 of 2,4-dichlorophenoxiacetic acid (2,4-D) combined with 2 mg · L−1 of benzyladenine (BA). Somatic embryos (SE) at the globular stage were encapsulated in a sodium alginate matrix; two treatments were tested: MS + 5 mg · L−1 BA + 1 mg · L−1 NAA + 3% (w/v) alginate, and MS + 7 mg · L−1 BA + 5.7 mg · L−1 indoleacetic acid (IAA) + 3% (w/v) alginate. Alginate was complexed with 100 mM calcium chloride (CaCl2). Viability of the encapsulated SE was determined by staining with 0.01% fluorescein diacetate (FDA) after 0, 15, 30, and 45 days of storage at 4°C. Embryo viability was 100% in both treatments.