Unique Features of a New Baeyer–Villiger Monooxygenase from a Halophilic Archaeon

Type I Baeyer–Villiger monooxygenases (BVMOs) are flavin-dependent monooxygenases that catalyze the oxidation of ketones to esters or lactones, a reaction otherwise performed in chemical processes by employing hazardous and toxic peracids. Even though various BVMOs are extensively studied for their...

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Bibliographic Details
Published in:Catalysts 2020-01, Vol.10 (1), p.128
Main Authors: Niero, Mattia, Righetto, Irene, Beneventi, Elisa, Polverino de Laureto, Patrizia, Fraaije, Marco Wilhelmus, Filippini, Francesco, Bergantino, Elisabetta
Format: Article
Language:English
Subjects:
Adaptation
Catalysts
Chemical reactions
Dehydrogenases
E coli
Enzymes
Esters
Genomes
Industrial applications
Industrial engineering
Ketones
Lactones
Manufacturing engineering
Microorganisms
Oxidation
Peracids
Polypeptides
Proteins
Salt
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Summary:Type I Baeyer–Villiger monooxygenases (BVMOs) are flavin-dependent monooxygenases that catalyze the oxidation of ketones to esters or lactones, a reaction otherwise performed in chemical processes by employing hazardous and toxic peracids. Even though various BVMOs are extensively studied for their promising role in industrial biotechnology, there is still a demand for enzymes that are able to retain activity at high saline concentrations. To this aim, and based on comparative in silico analyses, we cloned HtBVMO from the extremely halophilic archaeon Haloterrigena turkmenica DSM 5511. When expressed in standard mesophilic cell factories, proteins adapted to hypersaline environments often behave similarly to intrinsically disordered polypeptides. Nevertheless, we managed to express HtBVMO in Escherichia coli and could purify it as active enzyme. The enzyme was characterized in terms of its salt-dependent activity and resistance to some water–organic-solvent mixtures. Although HtBVMO does not seem suitable for industrial applications, it provides a peculiar example of an alkalophilic and halophilic BVMO characterized by an extremely negative charge. Insights into the behavior and structural properties of such salt-requiring may contribute to more efficient strategies for engineering the tuned stability and solubility of existing BVMOs.
ISSN:2073-4344
2073-4344
DOI:10.3390/catal10010128