Polymerically modified superparamagnetic iron oxide nanoparticles as a multi-model molecular probe for functionalized optical coherence tomography

• Published in: Optics and laser technology 2021-09, Vol.141, p.107108, Article 107108
• Main Authors: Indoliya, Abhishek, Mohan, Muktesh, Kharey, Prashant, Gupta, Sharad, Poddar, Raju
• Format: Article
• Language: English
• Subjects: Algorithms; Chemical synthesis; Contrast agents; Excitation; Frequency modulation; Iron oxides; Magnetic nanoparticles; Magnetic properties; Magnetomotive optical coherence tomography (MMOCT); Nano-scale displacement; Nanoparticles; Optical Coherence Tomography; Photothermal optical coherence tomography (PTOCT); Polyethylene coated Superparamagnetic iron oxide nanoparticles (PEG-SPIONs); Signal processing; Tomography
• ISSN: 0030-3992; 1879-2545
• DOI: 10.1016/j.optlastec.2021.107108

[Display omitted] •A high-speed multimodal molecular imaging is demonstrated based on OCT system.•A new spectroscopic MMOCT and PTOCT method was developed.•PEG-SPIONs were reported as multimodal contrast agent for OCT.•The system was validated with in-vivo imaging of PEG-SPIONs in mice model.•A penetration dynamic of PEG-SPIONs in tissue was also demonstrated. We demonstrate the synthesis, characterization, and potential application of polymerically modified superparamagnetic nanoparticles over bare SPIONs, as a multi-model molecular probe for functionalized optical coherence tomography (fOCT). The synthesized magnetic nanoparticles (MNPs) also have the ability of photo thermionic excitation under near-infrared irradiation (980 nm). By having dual excitation properties, the SPIONs are used as exogenous contrast agents for magnetomotive optical coherence tomography (MMOCT) as well as photothermal optical coherence tomography (PTOCT). We developed a functionalized OCT system and signal processing algorithms for MMOCT and PTOCT. Further, non-invasive in-vivo imaging was performed on swiss-albino mice to demonstrate the potential application of polymerically modified SPIONs. A diffusion dynamics study and contrast-to-noise ratio (CNR) study of the dispersed synthesized SPIONs inside skin tissue of mice as a function of time and depth is also reported. We found that PEG-SPIONs are excellent candidate for molecular contrast agent of functional OCT.