Baicalein-Enriched Fraction Extracted from Oroxylum indicum (L.) Benth. ex Kurz Leaves Exerts Antioxidant and Inhibitory Effects Against Glioblastoma Multiforme

Glioblastoma multiforme (GBM) is the most malignant subtype of primary brain cancer. To date, standard clinical treatment for GBM is limited in effectiveness and could impose additional side effects. Recently, numerous bioactive compounds isolated from natural plants appear to have beneficial anti-c...

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Bibliographic Details
Published in:Processes 2019-12, Vol.7 (12), p.963
Main Authors: Kang, In Nee, Nik Salleh, Nik Nur Hakimah, Chung, Wan Jie, Lee, Chong Yew, Tan, Suat Cheng
Format: Article
Language:English
Subjects:
Anticancer properties
Antioxidants
Bioactive compounds
Biological activity
Brain cancer
Cancer therapies
Cell cycle
Cell proliferation
Enrichment
Extraction procedures
Flavonoids
Fractionation
Glioblastoma
Glioblastoma multiforme
Leaves
Liver cancer
Metabolites
Methanol
Oroxylum indicum
Petroleum ether
Phytochemicals
Radiation therapy
Side effects
Solvents
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Summary:Glioblastoma multiforme (GBM) is the most malignant subtype of primary brain cancer. To date, standard clinical treatment for GBM is limited in effectiveness and could impose additional side effects. Recently, numerous bioactive compounds isolated from natural plants appear to have beneficial anti-cancer properties. Here, the GBM inhibitory effect of baicalein, a bioactive flavonoid extracted from Oroxylum indicum (L.) Benth. ex Kurz, was evaluated. Firstly, three solvents were used to extract the baicalein. We found that the binary extraction system, using a combination of petroleum ether and methanol (PM), yielded the highest amount of baicalein (15%) compared to the mono extraction system using methanol (13%) or aqueous (0.04%) only. In order to further enhance the baicalein yield in PM crude extract, it was subjected to an enrichment fractionation procedure, which successfully increased the baicalein by nearly two-fold from the initial crude extract (15%) to the enriched fraction 5 (F5) (29%). The enriched F5 not only showed significantly higher (~2.5-fold) antioxidant properties as compared to the crude extract, it was also found to significantly suppress GBM cell proliferation ~2.5-fold better than the crude extract. In conclusion, this study successfully optimized an extraction procedure for increased yield of baicalein metabolite from O. indicum leaves and enhanced its therapeutic potential for GBM treatment.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr7120963