Injectable Chitosan Scaffolds with Calcium β-Glycerophosphate as the Only Neutralizing Agent

The presented work describes the method of preparation of thermosensitive chitosan hydrogels using calcium β-glycerophosphate salt as the only pH neutralizing agent and supporting the crosslinking process. The presence of calcium ions instead of sodium ions is particularly important in the case of s...

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Bibliographic Details
Published in:Processes 2019-05, Vol.7 (5), p.297
Main Authors: Owczarz, Piotr, Rył, Anna, Dziubiński, Marek, Sielski, Jan
Format: Article
Language:English
Subjects:
b-Glycerophosphoric acid
Body temperature
Bones
Calcium carbonate
Calcium ions
Calcium phosphates
Chitosan
Crosslinking
Electrophoresis
Glycerophosphate
Hydrogels
Hydroxyapatite
Ions
Mechanical properties
Neutralizing
Phase transitions
Phosphorus
Physicochemical properties
Polymers
Rheological properties
Rheometry
Scaffolds
Sodium
Sol-gel processes
Tissue engineering
Viscosity
X ray photoelectron spectroscopy
Zeta potential
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Summary:The presented work describes the method of preparation of thermosensitive chitosan hydrogels using calcium β-glycerophosphate salt as the only pH neutralizing agent and supporting the crosslinking process. The presence of calcium ions instead of sodium ions is particularly important in the case of scaffolds in bone tissue engineering. Rheological and physicochemical properties of low concentrated chitosan solutions with the addition of calcium β-glycerophosphate were investigated using rotational rheometry techniques, Zeta potential (by electrophoresis), XPS, and SEM analysis together with an EDS detector. It was found to be possible to prepare colloidal solutions of chitosan containing only calcium β-glycerophosphate (without sodium ions) undergoing a sol-gel phase transition at the physiological temperature of the human body. It has also been shown that it is possible to further enrich the obtained cellular scaffolds with calcium ions. Using the addition of calcium carbonate, hydrogels with a physiological ratio of calcium to phosphorus (1.6–1.8):1 were obtained.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr7050297