An antifouling polymer for dynamic anti-protein adhesion analysis by a quartz crystal microbalance

Nowadays, the non-specific adsorption of biomolecules is a key issue in numerous fields. Herein, an improved antifouling molecule was synthesized by grafting phenol with oligopoly (ethylene glycol), named (4-(2-(2-ethoxyethoxy) ethoxy) phenol (EEP). An ideal antifouling polymer coating (PEEP) was sy...

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Bibliographic Details
Published in:Analyst (London) 2021-07, Vol.146 (14), p.4636-4641
Main Authors: Chen, Ximing, Ma, Yan, Gui, Qingwen, Hu, Shiyu, Pan, Weisong, Lan, Yaqin, Zeng, Mei, Zhou, Tiean, Su, Zhaohong
Format: Article
Language:English
Subjects:
Adhesion
Antifouling coatings
Biomedical materials
Biomolecules
Biosensors
Chemical synthesis
Ethylene glycol
Fourier transforms
Infrared spectrophotometers
Microbalances
Phenols
Polymer coatings
Polymerization
Polymers
Proteins
Quartz
Quartz crystals
Serum albumin
Spectrophotometry
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Summary:Nowadays, the non-specific adsorption of biomolecules is a key issue in numerous fields. Herein, an improved antifouling molecule was synthesized by grafting phenol with oligopoly (ethylene glycol), named (4-(2-(2-ethoxyethoxy) ethoxy) phenol (EEP). An ideal antifouling polymer coating (PEEP) was synthesized by the mechanism of electropolymerization of phenol. Quartz crystal microbalance (QCM), a sensitive mass sensor, was used to dynamically monitor both the modification and anti-protein adhesion (with bovine serum albumin as the model) process. Quantitatively, less proteins were observed to adhere to the modified electrode (277.8 ng for bare GCE and 8.88 ng for the modified GCE). Fourier transform infrared spectrophotometry (FT-IR), scanning electron microscopy (SEM), and electrochemical methods were used to study the coatings in detail. In this study, EEP was synthesized for the electrochemical preparation of an antifouling coating and characterized by QCM and electrochemical methods. The mild preparation environment (lower potential window and in phosphate buffered saline) and one-step method enable potential applications of PEEP in the field of biomaterials and biosensors. Quartz crystal microbalance was used to dynamically monitor both the modification and anti-protein adhesion process of an antifouling polymer coating.
ISSN:0003-2654
1364-5528
DOI:10.1039/d1an00856k