Infrared Emission Spectroscopy for Investigation of Biological Molecules in Aqueous Solutions

Examples are given of using infrared emission spectroscopy to analyze the structure of biological molecules in aqueous solutions. Instead of sample heating commonly used in emission spectroscopy, it is proposed to use a cold background for screening environmental thermal emission. Emission spectra a...

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Bibliographic Details
Published in:Physics of wave phenomena 2021-04, Vol.29 (2), p.164-168
Main Authors: Penkov, N. V., Penkova, N. A.
Format: Article
Language:English
Subjects:
Acoustics
Adenosine
Aqueous solutions
Aqueous Solutions in Biological Systems
Emission analysis
Emission spectra
Emission spectroscopy
Heating
Infrared analysis
Infrared spectroscopy
Liquid crystals
Molecular structure
Nucleotides
Phospholipids
Physics
Physics and Astronomy
Quantum Optics
Serum albumin
Spectrum analysis
Thermal emission
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Summary:Examples are given of using infrared emission spectroscopy to analyze the structure of biological molecules in aqueous solutions. Instead of sample heating commonly used in emission spectroscopy, it is proposed to use a cold background for screening environmental thermal emission. Emission spectra are measured for three types of molecular biological objects in aqueous solutions: protein (bovine serum albumin 1 mg/mL in H 2 O), phospholipid (1,2-dimiristoylphosphatidylcholine 1 mg/mL in D 2 O) in two phase states (gel at 10°С and liquid crystal at 35°С), and nucleotide (adenosine-5'-triphosphate 1 mg/mL in H 2 O). Characteristic bands are obtained for each of the samples. Higher sensitivity and wider application of infrared emission spectroscopy with a cold background in comparison to that with sample heating are discussed.
ISSN:1541-308X
1934-807X
DOI:10.3103/S1541308X21020102